PMID: 8995622Feb 1, 1997Paper

Functional identification of nucleotides conferring substrate specificity to retroviral integrase reactions

Journal of Virology
M Balakrishnan, C B Jonsson

Abstract

The long terminal repeats (LTRs) that flank the retroviral DNA genome play a distinct role in the integration process by acting as specific substrates for the integrase (IN). The role of LTR sequences in providing substrate recognition and specificity to integration reactions was investigated for INs from human immunodeficiency virus type 1 (HIV-1), Moloney murine leukemia virus (M-MuLV), human T-cell leukemia virus type 1 (HTLV-1), and human T-cell leukemia virus type 2 (HTLV-2). Overall, these INs required specific LTR sequences for optimal catalysis of 3'-processing reactions, as opposed to strand transfer and disintegration reactions. It is of particular note that in strand transfer reactions the sites of integration were similar among the four INs. In the 3'-processing reaction, sequence specificity for each IN was traced to the three nucleotides proximal to the conserved CA. Reactions catalyzed by M-MuLV IN were additionally influenced by upstream regions. The nucleotide requirements for optimal catalysis differed for each IN. HIV-1 IN showed a broad range of substrate specificities, while HTLV-1 IN and HTLV-2 IN had more defined sequence requirements. M-MuLV IN exhibited greater activity with the heterologous LTR substra...Continue Reading

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Citations

Nov 18, 2000·AIDS Research and Human Retroviruses·I LeclercqE Wattel
Jun 15, 2007·Journal of Virology·Sinu P JohnColleen B Jonsson
Feb 17, 2001·European Journal of Biochemistry·J SnáselI Pichová
Sep 28, 2000·The Journal of Biological Chemistry·E BrinJ Leis
Aug 25, 2009·Protein Science : a Publication of the Protein Society·Supachai SakkhachornphopChatchai Tayapiwatana
Jan 11, 2001·Journal of Virology·H ZhouJ M Coffin
Nov 21, 2019·Virology Journal·Fadila LarguetElsy Edouard
Dec 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Arash SoltaniBaratali Mashkani
Sep 3, 2019·Frontiers in Microbiology·Michał S BarskiGoedele N Maertens

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