PMID: 9160756May 16, 1997Paper

Functional identification of the mouse circadian Clock gene by transgenic BAC rescue

Cell
M P AntochJ S Takahashi

Abstract

As a complementary approach to positional cloning, we used in vivo complementation with bacterial artificial chromosome (BAC) clones expressed in transgenic mice to identify the circadian Clock gene. A 140 kb BAC transgene completely rescued both the long period and the loss-of-rhythm phenotypes in Clock mutant mice. Analysis with overlapping BAC transgenes demonstrates that a large transcription unit spanning approximately 100,000 base pairs is the Clock gene and encodes a novel basic-helix-loop-helix-PAS domain protein. Overexpression of the Clock transgene can shorten period length beyond the wild-type range, which provides additional evidence that Clock is an integral component of the circadian pacemaking system. Taken together, these results provide a proof of principle that "cloning by rescue" is an efficient and definitive method in mice.

References

Jul 10, 1992·Cell·A Bird
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·P E HardinM Rosbash
Aug 21, 1992·Cell·B Vogelstein, K W Kinzler
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H ShizuyaM Simon
Jan 1, 1990·Annual Review of Genetics·E J Stanbridge
Feb 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A J Levine
Jul 1, 1989·Physiological Reviews·J H Meijer, W J Rietveld
Jan 1, 1981·Molecular & General Genetics : MGG·R F Smith, R J Konopka
Jan 1, 1995·Annual Review of Neuroscience·J S Takahashi
Jul 1, 1993·Trends in Biotechnology·S Gibson, C Somerville
Jan 1, 1994·Recent Progress in Hormone Research·F W Turek
Jan 1, 1993·International Review of Cytology·G D BlockM Guesz
Jan 1, 1993·Annual Review of Physiology·C S Pittendrigh
Jan 1, 1993·Annual Review of Physiology·J C Dunlap
Jan 1, 1993·Annual Review of Physiology·J S TakahashiA Eskin
Jan 1, 1995·Methods in Cell Biology·A FavelloR K Wilson
Apr 19, 1996·Science·G Tosini, M Menaker
Jan 1, 1996·Annual Review of Genetics·J C Dunlap
Feb 1, 1997·Trends in Genetics : TIG·K R PetersonG Stamatoyannopoulos
Feb 1, 1997·Trends in Genetics : TIG·Y KashiM Soller
May 16, 1997·Cell·D P KingJ S Takahashi

❮ Previous
Next ❯

Citations

Jan 29, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M Hastings, E S Maywood
Dec 18, 2002·Journal of Neurobiology·Ralf Stanewsky
Nov 20, 2012·Cellular and Molecular Life Sciences : CMLS·Zheng ChenJoseph S Takahashi
Aug 10, 2006·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Jason D Heaney, Sarah K Bronson
Aug 13, 2011·Pflügers Archiv : European journal of physiology·Dominic LandgrafHenrik Oster
Apr 25, 2012·Brain Structure & Function·Martin F RathMorten Møller
Dec 7, 2010·Transgenic Research·Junko AsamiTakayoshi Inoue
Nov 19, 2003·European Journal of Pharmacology·David A Keays, Patrick M Nolan
Nov 19, 2003·European Journal of Pharmacology·Frauke Ohl, Martin E Keck
Aug 12, 1999·European Journal of Pharmacology·U Rudolph, H Möhler
Jan 7, 1999·Physiology & Behavior·M W HurdG M Cahill
Mar 23, 2000·Theriogenology·S M Kappes
Sep 1, 2000·Brain Research. Brain Research Reviews·K E van EsseveldtG J Boer
Nov 5, 1997·Trends in Neurosciences·M H Hastings
May 3, 2003·Trends in Biotechnology·Haydn Prosser, Sohaila Rastan
Nov 11, 1998·Neuroscience Research·K Kako, N Ishida
Jan 10, 1998·Trends in Genetics : TIG·E M Rubin, D J Smith
Oct 24, 2000·Brain Research. Molecular Brain Research·M ZhuangJ C Besharse
Jul 17, 1999·Brain Research. Molecular Brain Research·P LarkinS L Semple-Rowland
Feb 25, 2000·Brain Research. Molecular Brain Research·H ZhuC B Green
Dec 22, 1999·International Journal of Immunopharmacology·C HundeikerC Esser
May 25, 1999·Progress in Neurobiology·J Falcón
Nov 13, 2001·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·F W Turek, D E Kolker
May 10, 2001·Current Opinion in Cell Biology·J A Ripperger, U Schibler
Nov 13, 1998·Current Opinion in Neurobiology·D WhitmoreN S Foulkes
Nov 13, 1998·Current Opinion in Neurobiology·R Costa, C P Kyriacou
Mar 10, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·C FocanN Moeneclaey
Sep 25, 2001·Immunity·E K WakelandT W Behrens
May 7, 1998·The International Journal of Biochemistry & Cell Biology·S Morwenna, W P Ratcliffe
May 2, 2013·European Journal of Human Genetics : EJHG·Klaus SchughartMartien J Kas
Jul 15, 2009·International Journal of Obesity : Journal of the International Association for the Study of Obesity·X WuJ M Gimble
Feb 21, 2007·Nature Cell Biology·Wen-Ning ZhaoCharles J Weitz
Aug 30, 2001·Nature Immunology·A Wandstrat, E Wakeland
Oct 29, 2004·Nature Reviews. Genetics·Stylianos E AntonarakisSamuel Deutsch

❮ Previous
Next ❯

Related Concepts

Related Feeds

Artificial Chromosomes

Artificial chromosomes are genetically engineered chromosomes derived from the DNA of a species. Discover the latest research on artificial chromosomes here.

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.