Functional identification of the Proteus mirabilis core lipopolysaccharide biosynthesis genes.

Journal of Bacteriology
Eleonora AquiliniMiguel Regué

Abstract

In this study, we report the identification of genes required for the biosynthesis of the core lipopolysaccharides (LPSs) of two strains of Proteus mirabilis. Since P. mirabilis and Klebsiella pneumoniae share a core LPS carbohydrate backbone extending up to the second outer-core residue, the functions of the common P. mirabilis genes was elucidated by genetic complementation studies using well-defined mutants of K. pneumoniae. The functions of strain-specific outer-core genes were identified by using as surrogate acceptors LPSs from two well-defined K. pneumoniae core LPS mutants. This approach allowed the identification of two new heptosyltransferases (WamA and WamC), a galactosyltransferase (WamB), and an N-acetylglucosaminyltransferase (WamD). In both strains, most of these genes were found in the so-called waa gene cluster, although one common core biosynthetic gene (wabO) was found outside this cluster.

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Citations

Oct 21, 2010·The Journal of Biological Chemistry·Eleonora AquiliniMiguel Regué
Feb 26, 2013·Journal of Bacteriology·Kristylea J OjedaK Dale Noel
Apr 24, 2014·International Journal of Molecular Sciences·Eleonora AquiliniJuan M Tomás
Jun 1, 2017·International Journal of Molecular Sciences·Marta KaszowskaJuan M Tomás
Nov 19, 2013·Journal of Bacteriology·Eleonora AquiliniJuan M Tomás

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