Functional identity of a purified proximal tubule anion exchanger protein: mediation of chloride/formate and chloride/bicarbonate exchange

Kidney International
M Soleimani, G L Bizal

Abstract

Based on the transport activities and inhibitor sensitivities, different functional modes of anion exchangers have been identified in the kidney proximal tubule including chloride/formate, chloride/oxalate, chloride/hydroxyl, and chloride/bicarbonate exchange. There is little information on the molecular structure and properties of the protein(s) involved in these processes. Previously, using stilbene affinity matrix and Pac Q chromatography, we partially purified a protein with anion exchange properties in brush border membranes (BBM) isolated from rabbit kidney proximal tubules. This protein has a molecular weight of 162 kDa. When reconstituted into liposomes, the fraction containing the 162 kDa protein demonstrated Cl-/Cl- exchange activity. In the current experiments, the 162 kDa protein was purified to homogeneity using a combination of affinity, ion exchange, and size exclusion chromatography. This protein has binding affinity for known inhibitors of anion exchangers. When reconstituted in liposomes, the 162 kDa protein showed anion exchange activity as assayed by 36Cl-/Cl- exchange. Functional studies in liposomes reconstituted with the purified 162 kDa protein in revealed that this protein mediates the transport of Cl-/...Continue Reading

References

Jan 1, 1991·Annual Review of Physiology·S L Alper
Jun 1, 1989·The Journal of Clinical Investigation·P A Preisig, R J Alpern
Apr 1, 1987·The Journal of Clinical Investigation·R J Alpern
Dec 11, 1987·Biochimica Et Biophysica Acta·S M GrasslC R Ross
Dec 1, 1986·Biochimica Et Biophysica Acta·H E IvesA S Verkman
Jan 1, 1987·The Journal of Clinical Investigation·L SchildP S Aronson
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·L P Karniski, P S Aronson
Sep 1, 1993·Kidney International·M SoleimaniC C Anderson
Jan 1, 1994·Circulation Research·M Soleimani, R L Howard

❮ Previous
Next ❯

Citations

Nov 18, 1998·Current Opinion in Nephrology and Hypertension·F Roch-Ramel
Aug 10, 2001·Current Opinion in Nephrology and Hypertension·M Soleimani
May 18, 1999·Pharmacology & Toxicology·J S Petersen
Jul 31, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lara R GawenisLane L Clarke
Oct 17, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Snezana PetrovicManoocher Soleimani
Feb 24, 2001·American Journal of Physiology. Renal Physiology·M SoleimaniC E Burnham

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.