DOI: 10.1101/506774Dec 27, 2018Paper

Functional imaging of microbial interactions with tree roots using a microfluidics setup

BioRxiv : the Preprint Server for Biology
Marie-Francois S GrosGyorgy Babnigg

Abstract

Coupling microfludics with microscopy has emerged as a powerful approach to study dynamics in plant physiology at cellular resolution and at higher throughput than with conventional methods. Most devices have been designed to study the model plant Arabidopsis thaliana, including root-microbe interactions. However, there is a need for microfluidic devices which enable in vivo studies of root development and root-microbe interactions in woody plants. Here, we developed the RMI-chip, a microfluidic setup that allows for continuous microscopic scale observation of real-time interactions between live Populus tremuloides (aspen tree) seedling roots and the rhizobacterium Pseudomonas fluorescens over an extended period longer than a month. Colonization dynamics in the RMI-chip are consistent with previous observations in a different experimental set-up. Also, we find that biosensors based on the rhizobacterium Bacillus subtilis can be used to monitor compositional changes in root exudates but that their application is limited by their inefficient colonization of aspen roots. Our results indicate that functional imaging of dynamic root-bacteria interactions in the RMI-chip requires careful matching between the host plant and the bacter...Continue Reading

Related Concepts

Diagnostic Imaging
Medical Devices
Microscopy
Physiological Aspects
Rhizobium
Trees (plant)
Arabidopsis thaliana <plant>
Disks (Device)
Seedling
Plant Roots

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