Functional Impact of the H2A.Z Histone Variant During Meiosis in Saccharomyces cerevisiae

Genetics
Sara González-ArranzPedro A San-Segundo

Abstract

Among the collection of chromatin modifications that influence its function and structure, the substitution of canonical histones by the so-called histone variants is one of the most prominent actions. Since crucial meiotic transactions are modulated by chromatin, here we investigate the functional contribution of the H2A.Z histone variant during both unperturbed meiosis and upon challenging conditions where the meiotic recombination checkpoint is triggered in budding yeast by the absence of the synaptonemal complex component Zip1 We have found that H2A.Z localizes to meiotic chromosomes in an SWR1-dependent manner. Although meiotic recombination is not substantially altered, the htz1 mutant (lacking H2A.Z) shows inefficient meiotic progression, impaired sporulation, and reduced spore viability. These phenotypes are likely accounted for by the misregulation of meiotic gene expression landscape observed in htz1 In the zip1 mutant, the absence of H2A.Z results in a tighter meiotic arrest imposed by the meiotic recombination checkpoint. We have found that Mec1-dependent Hop1-T318 phosphorylation and the ensuing Mek1 activation are not significantly altered in zip1 htz1; however, downstream checkpoint targets, such as the meiosis I...Continue Reading

References

Oct 23, 1998·Science·S ChuI Herskowitz
May 13, 1999·Cell·P A San-Segundo, G S Roeder
Oct 12, 2000·Molecular Biology of the Cell·P A San-Segundo, G S Roeder
Oct 18, 2000·Proceedings of the National Academy of Sciences of the United States of America·K S TungG S Roeder
Jun 5, 2003·Genes & Development·Kirsten R BenjaminIra Herskowitz
Dec 24, 2003·Molecular Cell·Nevan J KroganJack F Greenblatt
May 29, 2004·Genetics·Eric J WhiteDavid B Kaback
Aug 13, 2005·Cell·Stacy L HarveyDouglas R Kellogg
Oct 14, 2005·Molecular Biology of the Cell·Hengyao NiuNancy M Hollingsworth
Jul 29, 2006·Cell·Emmanuelle MartiniScott Keeney
Oct 4, 2008·Genes & Development·Anuradha Sourirajan, Michael Lichten
Aug 18, 2009·Methods in Molecular Biology·Beth Rockmill
Nov 18, 2009·Molecular Cell·Hengyao NiuNancy M Hollingsworth
Apr 27, 2011·The Journal of Cell Biology·Jennifer M GardnerSue L Jaspersen
May 3, 2011·Trends in Cell Biology·Amy J MacQueen, Andreas Hochwagen
Jun 23, 2011·Journal of Cell Science·Esther RefolioPedro A San-Segundo
Jul 29, 2011·Molecular Biology of the Cell·Isabel AcostaPedro A San-Segundo
Dec 23, 2011·Biology of the Cell·Elsa BrachetValérie Borde
Mar 7, 2012·Microbiology and Molecular Biology Reviews : MMBR·Edward Winter
Aug 14, 2012·Nature Structural & Molecular Biology·Kiran MahajanNupam P Mahajan
Oct 17, 2012·PLoS Genetics·Karen Voelkel-MeimanAmy J MacQueen

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Citations

Nov 17, 2020·Frontiers in Cell and Developmental Biology·Sara González-ArranzPedro A San-Segundo
Sep 12, 2020·Current Biology : CB·Vivek B Raina, Gerben Vader
Jul 15, 2021·PLoS Genetics·Esther HerruzoPedro A San-Segundo

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