Functional impairment of nigrostriatal neurons progresses following withdrawal of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine

Neuroscience
A AlbaneseP Tonali

Abstract

C57 BL/6 mice were rendered severely parkinsonian by exposure to high doses of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. The fluorescent retrograde tracer Fast Blue was injected into the neostriatum one (group A) or five weeks (group B) following exposure to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. Neurons located in the substantia nigra pars compacta and in the centre median-parafascicular complex were analysed. There was no variation in the number and distribution of Fast Blue-labelled perikarya located in the centre median-parafascicular complex, which are insensitive to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. No variation was seen in the number of Nissl-stained perikarya located in the substantia nigra pars compacta, indicating that neurons had not degenerated. The number and the density of Fast Blue retrogradely-labelled neurons located in the same region were decreased in group A by 41% and in group B by 55%. Fast Blue labelling provided a measure of functional impairment in viable neurons. The Fast Blue-to-Nissl cell ratio was 55% in controls and declined to 20% in group A and to 17% in group B mice. The present study shows that (1) functional inactivation of viable neurons can be measured by using a fluor...Continue Reading

References

Sep 8, 1990·The Journal of Comparative Neurology·H W Berendse, H J Groenewegen
Jul 1, 1982·Journal of Neuroscience Methods·A Albanese, M Bentivoglio
Nov 1, 1977·Neuroscience Letters·H G KuypersR E Padt

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Citations

Mar 26, 1999·Movement Disorders : Official Journal of the Movement Disorder Society·E Moro, A Albanese
Jul 24, 2008·Journal of Neuropathology and Experimental Neurology·Alison L McCormackDonato A Di Monte

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