PMID: 11923096Mar 30, 2002Paper

Functional implications of post-translational modifications of phospholipases D1 and D2

Biochimica Et Biophysica Acta
Zhi XieJohn H Exton

Abstract

Our previous studies showed that truncation of the N-terminal 168 amino acids of rat brain phospholipase D1 (rPLD1) abolishes its response to protein kinase C (PKC) and greatly diminishes its palmitoylation and Ser/Thr phosphorylation. In this study, we show that the response to PKC as well as the palmitoylation and Ser/Thr phosphorylation were restored when the truncated rPLD1 mutant (rPLD1(DeltaN168)) was coexpressed with a fragment containing the N-terminal 168 amino acids. Immunoprecipitation experiments showed that the N-terminal fragment associated with rPLD1(DeltaN168) when coexpressed in COS 7 cells and that palmitoylation of Cys(240) and Cys(241) was not necessary for the association. In addition, we found that rat PLD2 (rPLD2) was palmitoylated on Cys(223) and Cys(224) in COS 7 cells. Mutation of both these cysteines reduced the basal activity of rPLD2, however its response to PMA stimulation in vivo was retained. As in the case of rPLD1, loss of palmitoylation weakened membrane association of rPLD2. In summary, the N-terminal 168-amino-acid fragment of rPLD1 can associate with truncated rPLD1(DeltaN168) to restore its palmitoylation, Ser/Thr phosphorylation and PKC response. Although rPLD2 differs from rPLD1 in many ...Continue Reading

References

May 1, 1996·Trends in Pharmacological Sciences·A J MorrisM A Frohman
Apr 25, 1997·The Journal of Biological Chemistry·T Kodaki, S Yamashita
Dec 18, 1998·The Journal of Biological Chemistry·Z XieJ H Exton
Jan 28, 1999·The Journal of Biological Chemistry·T C SungM A Frohman
Nov 18, 2000·The Journal of Biological Chemistry·M Y LiuP C Sternweis
Dec 31, 2000·The Journal of Biological Chemistry·Z XieJ H Exton

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Citations

Jan 14, 2004·Molecular Biology of the Cell·Guangwei DuMichael A Frohman
Sep 23, 2011·Chemical Reviews·Paige E SelvyH Alex Brown
Jun 11, 2004·Annual Review of Biochemistry·Jessica E Smotrys, Maurine E Linder
Aug 22, 2003·Protein Science : a Publication of the Protein Society·Hongying Yang, Mary F Roberts
Sep 25, 2004·Journal of Cellular Physiology·Masami Hiroyama, John H Exton
Dec 16, 2016·Nature Communications·E Nicholas PetersenScott B Hansen
Jun 2, 2018·Journal of Lipid Research·Antonio Luis Egea-Jimenez, Pascale Zimmermann
Apr 7, 2004·The Journal of Biological Chemistry·Akiko KageyamaChikako Nishigori
Sep 24, 2014·Pharmacological Reviews·Ronald C BruntzH Alex Brown
Nov 2, 2019·Biochimica Et Biophysica Acta. Biomembranes·E Nicholas PetersenScott B Hansen
Dec 13, 2019·Progress in Lipid Research·M I McDermottV A Bankaitis

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