PMID: 9531480Jun 11, 1998Paper

Functional implications of ribosomal protein L2 in protein biosynthesis as shown by in vivo replacement studies

The Biochemical Journal
M UhleinB Wittmann-Liebold

Abstract

The translational apparatus is a highly complex structure containing three to four RNA molecules and more than 50 different proteins. In recent years considerable evidence has accumulated to indicate that the RNA participates intensively in the catalysis of peptide-bond formation, whereas a direct involvement of the ribosomal proteins has yet to be demonstrated. Here we report the functional and structural conservation of a peptidyltransferase centre protein in all three phylogenetic domains. In vivo replacement studies show that the Escherichia coli L2 protein can be replaced by its homologous proteins from human and archaebacterial ribosomes. These hybrid ribosomes are active in protein biosynthesis, as proven by polysome analysis and poly(U)-dependent polyphenylalanine synthesis. Furthermore, we demonstrate that a specific, highly conserved, histidine residue in the C-terminal region of L2 is essential for the function of the translational apparatus. Replacement of this histidine residue in the human and archaebacterial proteins by glycine, arginine or alanine had no effect on ribosome assembly, but strongly reduced the translational activity of ribosomes containing these mutants.

Citations

Aug 24, 2010·Biochemical and Biophysical Research Communications·Yuko Motojima-MiyazakiFumihiro Motojima
Oct 6, 2000·Annual Review of Microbiology·M Riley, M H Serres
Feb 12, 2008·Nucleic Acids Research·Arturas MeskauskasJonathan D Dinman
Mar 13, 2009·Nucleic Acids Research·Chiaolong Hsiao, Loren Dean Williams
Dec 20, 2012·MBio·Josephine R ChandlerE Peter Greenberg
Mar 17, 2018·Biochemistry. Biokhimii︠a︡·A D Nikulin
Apr 11, 2006·Genome Génome / Conseil National De Recherches Canada·Selvi Subramanian, Linda Bonen
May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·A BashanA Yonath
Aug 2, 2001·Biochemical and Biophysical Research Communications·K MikulíkP Halada
Dec 15, 2000·Journal of Molecular Biology·R WillumeitK H Nierhaus
Aug 30, 2003·Journal of Molecular Biology·Fotini LeontiadouTheodora Choli-Papadopoulou
Jun 29, 2021·Frontiers in Microbiology·Sheeba Santhini Manoharan-BasilChris Kenyon
Apr 3, 2001·Science·M M YusupovH F Noller

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