Functional integration of human neural precursor cells in mouse cortex

PloS One
Fu-Wen ZhouSteven N Roper

Abstract

This study investigates the electrophysiological properties and functional integration of different phenotypes of transplanted human neural precursor cells (hNPCs) in immunodeficient NSG mice. Postnatal day 2 mice received unilateral injections of 100,000 GFP+ hNPCs into the right parietal cortex. Eight weeks after transplantation, 1.21% of transplanted hNPCs survived. In these hNPCs, parvalbumin (PV)-, calretinin (CR)-, somatostatin (SS)-positive inhibitory interneurons and excitatory pyramidal neurons were confirmed electrophysiologically and histologically. All GFP+ hNPCs were immunoreactive with anti-human specific nuclear protein. The proportions of PV-, CR-, and SS-positive cells among GFP+ cells were 35.5%, 15.7%, and 17.1%, respectively; around 15% of GFP+ cells were identified as pyramidal neurons. Those electrophysiologically and histological identified GFP+ hNPCs were shown to fire action potentials with the appropriate firing patterns for different classes of neurons and to display spontaneous excitatory and inhibitory postsynaptic currents (sEPSCs and sIPSCs). The amplitude, frequency and kinetic properties of sEPSCs and sIPSCs in different types of hNPCs were comparable to host cells of the same type. In conclusio...Continue Reading

References

Feb 16, 1996·Science·C LoisA Alvarez-Buylla
Jun 1, 1997·Cerebral Cortex·Y Gonchar, A Burkhalter
Sep 1, 1997·Cerebral Cortex·Y Kawaguchi, Y Kubota
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·S LiuJ W McDonald
Jul 7, 2000·Molecular and Cellular Neurosciences·F J RubioA Martínez-Serrano
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·N UchidaI L Weissman
Apr 12, 2002·Brain Research. Developmental Brain Research·M A AleksandrovaG T Sukhikh
Jul 12, 2002·Current Gene Therapy·L J Chang, E E Gay
Dec 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ulrica EnglundMerab Kokaia
Apr 23, 2003·Brain Research·Mark A LikerMichael W Jakowec
Dec 25, 2003·Epilepsia·Kerry W Thompson, Lucie M Suchomelova
Jun 4, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Marius WernigOliver Brüstle
Jul 29, 2004·Proceedings of the National Academy of Sciences of the United States of America·S KellyG K Steinberg
Sep 21, 2004·Nature Reviews. Neuroscience·Henry MarkramCaizhi Wu
May 13, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hans S KeirsteadOswald Steward
Sep 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Brian J CummingsAileen J Anderson
Apr 28, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sanjay S Kumar, Paul S Buckmaster
Jul 14, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Manuel Alvarez-DoladoScott C Baraban
Jan 11, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Fu-Wen ZhouFu-Ming Zhou
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Rutger-Jan SwijnenburgJoseph C Wu
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Scott C BarabanArturo Alvarez-Buylla
Jan 22, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Makoto IdeguchiJames M Weimann
Sep 10, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Fuzheng GuoDavid Pleasure
Nov 19, 2010·Cerebral Cortex·Fu-Wen Zhou, Steven N Roper
Jan 6, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xu MaisanoJanice R Naegele

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Citations

Feb 4, 2016·Stem Cells International·Sára KálmánJános M Réthelyi
Apr 1, 2016·Scientific Reports·Jeff M FortinBrent A Reynolds
May 27, 2016·Journal of Neurophysiology·MacKenzie A Howard, Scott C Baraban
Mar 15, 2019·Stem Cell Research & Therapy·Dasa BohaciakovaMartin Marsala

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Methods Mentioned

BETA
Assay
fluorescence microscopy
xenograft
xenografts

Software Mentioned

ImageJ
MultiClamp
pClamp
MiniAnalysis

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