Functional interaction between bovine rhodopsin and G protein transducin

The Journal of Biological Chemistry
Akihisa TerakitaYoshinori Shichida

Abstract

To elucidate the mechanisms of specific coupling of bovine rhodopsin with the G protein transducin (G(t)), we have constructed the bovine rhodopsin mutants whose second or third cytoplasmic loop (loop 2 or 3) was replaced with the corresponding loop of the G(o)-coupled scallop rhodopsin and investigated the difference in the activation abilities for G(t), G(o), and G(i) among these mutants and wild type. We have also prepared the Galpha(i) mutants whose C-terminal 11 or 5 amino acids were replaced with those of Galpha(t), Galpha(o), and Galpha(q) to evaluate the role of the C-terminal tail of the alpha-subunit on the specific coupling of bovine rhodopsin with G(t). Replacement of loop 2 of bovine rhodopsin with that of the scallop rhodopsin caused about a 40% loss of G(t) and G(o) activation, whereas that of loop 3 enhanced the G(o) activation four times with a 60% decrease in the G(t) activation. These results indicated that loop 3 of bovine rhodopsin is one of the regions responsible for the specific coupling with G(t). Loop 3 of bovine rhodopsin discriminates the difference of the 6-amino acid sequence (region A) at a position adjacent to the C-terminal 5 amino acids of the G protein, resulting in the different activation ef...Continue Reading

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Citations

Dec 8, 2010·Proceedings of the National Academy of Sciences of the United States of America·Takahiro YamashitaYoshinori Shichida
Mar 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Mitsumasa KoyanagiAkihisa Terakita
Mar 28, 2008·Photochemistry and Photobiology·Takahiro YamashitaYoshinori Shichida
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