Functional interaction between JC virus late regulatory agnoprotein and cellular Y-box binding transcription factor, YB-1

Journal of Virology
Mahmut SafakKamel Khalili

Abstract

Human polyomavirus JC virus (JCV) is a causative agent of progressive multifocal leukoencephalopathy which results from lytic infection of glial cells. Although significant progress has been made in understanding the regulation of JCV gene transcription, the mechanism(s) underlying the viral lytic cycle remains largely unknown. We recently reported that the JCV late auxiliary Agnoprotein may have a regulatory role in JCV gene transcription and replication. Here, we investigated its regulatory function in viral gene transcription through its physical and functional interaction with YB-1, a cellular transcription factor which contributes to JCV gene expression in glial cells. Time course studies revealed that Agnoprotein is first detected at day 3 postinfection and that its level increased during the late stage of the infection cycle. Agnoprotein is mainly localized to the cytoplasmic compartment of the infected cell, with high concentrations found in the perinuclear region. While the position of Agnoprotein throughout the infection cycle remained relatively unaltered, the subcellular distribution of YB-1 between the cytoplasm and nucleus changed. Results from coimmunoprecipitation and glutathione S-transferase pull-down experime...Continue Reading

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Citations

Apr 20, 2010·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Leslie J Marshall, Eugene O Major
Jul 31, 2009·Journal of Virology·Martyn K WhiteKamel Khalili
Dec 13, 2002·Journal of Virology·Beata SadowskaMahmut Safak
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Jul 29, 2004·Journal of Virology·Armine DarbinyanKamel Khalili
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Jul 24, 2008·Journal of Neuropathology and Experimental Neurology·Luis Del ValleKamel Khalili
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Feb 9, 2017·Infectious Agents and Cancer·Serena DelbuePasquale Ferrante

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