Functional interaction between paramyxovirus fusion and attachment proteins.

The Journal of Biological Chemistry
Jin K LeeRichard K Plemper

Abstract

Paramyxovirinae envelope glycoproteins constitute a premier model to dissect how specific and dynamic interactions in multisubunit membrane protein complexes can control deep-seated conformational rearrangements. However, individual residues that determine reciprocal specificity of the viral attachment and fusion (F) proteins have not been identified. We have developed an assay based on a pair of canine distemper virus (CDV) F proteins (strains Onderstepoort (ODP) and Lederle) that share approximately 95% identity but differ in their ability to form functional complexes with the measles virus (MV) attachment protein (H). Characterization of CDV F chimeras and mutagenesis reveals four residues in CDV F-ODP (positions 164, 219, 233, and 317) required for productive interaction with MV H. Mutating these residues to the Lederle type disrupts triggering of F-ODP by MV H without affecting functionality when co-expressed with CDV H. Co-immunoprecipitation shows a stronger physical interaction of F-ODP than F-Lederle with MV H. Mutagenesis of MV F highlights the MV residues homologous to CDV F residues 233 and 317 as determinants for physical glycoprotein interaction and fusion activity under homotypic conditions. In assay reversal, th...Continue Reading

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Citations

Feb 25, 2011·Expert Reviews in Molecular Medicine·Hector C Aguilar, Benhur Lee
Jan 11, 2011·Nature Structural & Molecular Biology·Chanakha K NavaratnarajahRoberto Cattaneo
Oct 3, 2012·Proceedings of the National Academy of Sciences of the United States of America·Melinda A BrindleyRichard K Plemper
Sep 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Brett D WelchRobert A Lamb
Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sayantan BoseRobert A Lamb
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Aug 13, 2010·Journal of Virology·Everett Clinton Smith, Rebecca Ellis Dutch
Aug 7, 2009·Journal of Virology·Tanja PaalRichard K Plemper
Jul 4, 2013·MBio·Philippe Plattet, Richard K Plemper
Jun 10, 2011·PLoS Pathogens·Richard K PlemperRonald M Iorio
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Nov 3, 2009·The FEBS Journal·Everett C SmithRebecca Ellis Dutch
Feb 5, 2014·Current Opinion in Virology·Mathieu MateoRoberto Cattaneo

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