Functional interaction of CP2 with GATA-1 in the regulation of erythroid promoters.

Molecular and Cellular Biology
Francesca BosèA Ronchi

Abstract

We observed that binding sites for the ubiquitously expressed transcription factor CP2 were present in regulatory regions of multiple erythroid genes. In these regions, the CP2 binding site was adjacent to a site for the erythroid factor GATA-1. Using three such regulatory regions (from genes encoding the transcription factors GATA-1, EKLF, and p45 NF-E2), we demonstrated the functional importance of the adjacent CP2/GATA-1 sites. In particular, CP2 binds to the GATA-1 HS2 enhancer, generating a ternary complex with GATA-1 and DNA. Mutations in the CP2 consensus greatly impaired HS2 activity in transient transfection assays with K562 cells. Similar results were obtained by transfection of EKLF and p45 NF-E2 mutant constructs. Chromatin immunoprecipitation with K562 cells showed that CP2 binds in vivo to all three regulatory elements and that both GATA-1 and CP2 were present on the same GATA-1 and EKLF regulatory elements. Adjacent CP2/GATA-1 sites may represent a novel module for erythroid expression of a number of genes. Additionally, coimmunoprecipitation and glutathione S-transferase pull-down experiments demonstrated a physical interaction between GATA-1 and CP2. This may contribute to the functional cooperation between the...Continue Reading

References

Feb 1, 1992·Molecular and Cellular Biology·L C LimM Sheffery
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·K OnoderaM Yamamoto
May 9, 1997·The Journal of Biological Chemistry·S TakahashiM Yamamoto
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·M A McDevittS H Orkin
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·M A McDevittS H Orkin
Jun 11, 1998·The Journal of Biological Chemistry·K P AndersonJ B Lingrel
Jul 25, 1998·Genes and Function·A RonchiS Ottolenghi
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·J S LeeJ H Chung
Sep 24, 1999·Biochemical and Biophysical Research Communications·J H ChaeC G Kim
May 16, 2000·The Journal of Biological Chemistry·D SeshasayeeD M Wojchowski
Sep 21, 2000·The Journal of Biological Chemistry·L RamamurthyS M Jane
Sep 26, 2000·Molecular and Cellular Biology·W ZhouS M Jane
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·T SawadoM Groudine
Sep 5, 2001·Current Opinion in Genetics & Development·A B Cantor, S H Orkin
Sep 6, 2001·Blood·H KawabataH P Koeffler
Sep 22, 2001·The EMBO Journal·M S RistaldiA M Imam
Dec 26, 2001·Nucleic Acids Research·N A KolchanovA G Romashchenko
Aug 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Kirby D JohnsonEmery H Bresnick
Feb 3, 2005·Molecular and Cellular Biology·Rita FerreiraSjaak Philipsen
Jul 1, 2005·Molecular and Cellular Biology·Ho Chul KangChul Geun Kim

❮ Previous
Next ❯

Citations

Feb 1, 2011·International Journal of Hematology·Mikiko SuzukiMasayuki Yamamoto
Aug 22, 2009·Evidence-based Complementary and Alternative Medicine : ECAM·Mika OhtaYutaka Takaoka
Sep 22, 2009·Nucleic Acids Research·Ying ZhangRoss C Hardison
Sep 21, 2010·Nucleic Acids Research·Claudio Cantu'Antonella Ronchi
Apr 13, 2012·Nucleic Acids Research·J MarianiS K Nicolis
Mar 19, 2014·International Journal of Hematology·Takashi Moriguchi, Masayuki Yamamoto
Sep 16, 2009·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Ayako KatsuraKazuhiro Sogawa
Oct 15, 2013·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Ritsuko ShimizuMasayuki Yamamoto
Sep 4, 2012·Cancer Science·Ritsuko Shimizu, Masayuki Yamamoto
Aug 9, 2012·Cell Cycle·Bárbara Fernández-MoralesCarmela Calés
Mar 29, 2008·Cell Stem Cell·Cristina PinaTariq Enver
Jan 26, 2011·Blood·Claudio CantùAntonella Ronchi
Nov 7, 2015·Evolutionary Bioinformatics Online·Gisele Cristine de Souza CarrociniClaudia Regina Bonini-Domingos
Jan 26, 2017·Frontiers in Oncology·Atsushi Hasegawa, Ritsuko Shimizu
Mar 17, 2018·Nucleic Acids Research·Min Young KimChul Geun Kim
Oct 22, 2020·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Simon Söderholm, Claudio Cantù
Aug 8, 2021·International Journal of Molecular Sciences·Chun-Hao HungChe-Kun James Shen

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.