Functional interaction of nicotinic acetylcholine receptors and Na+/K+ ATPase from Locusta migratoria manilensis (Meyen)

Scientific Reports
Haibo BaoZhong Li

Abstract

Associated proteins are important for the correct functioning of nicotinic acetylcholine receptors (nAChRs). In the present study, a neonicotinoid-agarose affinity column was used to isolate related proteins from a solubilized membrane preparation from the nervous system of Locusta migratoria manilensis (Meyen). 1530 peptides were identified and most of them were involved in the membranous structure, molecular interaction and cellular communication. Among these peptides, Na(+)/K(+) ATPase had the highest MASCOT score and were involved in the molecular interaction, which suggested that Na(+)/K(+) ATPase and nAChRs might have strong and stable interactions in insect central nervous system. In the present study, functional interactions between nAChRs and Na(+)/K(+) ATPase were examined by heterologous expression in Xenopus oocytes. The results showed that the activated nAChRs increased pump currents of Na(+)/K(+) ATPase, which did not require current flow through open nAChRs. In turn, Na(+)/K(+) ATPase significantly increased agonist sensitivities of nAChRs in a pump activity-independent manner and reduced the maximum current (Imax) of nAChRs. These findings provide novel insights concerning the functional interactions between ins...Continue Reading

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Citations

Jan 13, 2016·The Journal of General Physiology·Violetta V KravtsovaIgor I Krivoi
Jun 3, 2016·Frontiers in Physiology·Vladimir V Matchkov, Igor I Krivoi
Oct 30, 2016·Insect Biochemistry and Molecular Biology·Haibo BaoZhong Li
Mar 3, 2019·International Journal of Molecular Sciences·Igor I Krivoi, Alexey M Petrov

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Datasets Mentioned

BETA
KF813096
KF813099

Methods Mentioned

BETA
pulldown
interaction studies
Protein Assay
nuclear
NMR
electrophoresis

Software Mentioned

MASCOT

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