Functional interaction of Src family kinases with the acetylcholine receptor in C2 myotubes.

The Journal of Biological Chemistry
Christian Fuhrer, Z W Hall

Abstract

Tyrosine phosphorylation of the beta subunit of the acetylcholine receptor (AChR) has been postulated to play a role in AChR clustering during development of the neuromuscular junction. We have investigated the mechanism of this phosphorylation in mammalian C2 myotubes and report that the tyrosine kinase Src binds and phosphorylates glutathione S-transferase fusion proteins containing the N-terminal half of the cytoplasmic loop of the beta subunit. No binding occurs to the related kinases Fyn or Yes or to the corresponding regions from the gamma and delta subunits. Furthermore, AChRs affinity-isolated from C2 myotubes using alpha-bungarotoxin-Sepharose were specifically associated with Src and Fyn and had tyrosine-phosphorylated beta subunits. We suggest that AChRs are initially phosphorylated by Src and subsequently bind Fyn in a phosphotyrosine-dependent manner. These interactions are likely to play an important role in construction of the specialized postsynaptic membrane during synaptogenesis.

References

Jul 1, 1992·Journal of Neurobiology·B G Wallace
Jun 1, 1991·Current Opinion in Neurobiology·K R WagnerR L Huganir
Jan 1, 1991·Annual Review of Pharmacology and Toxicology·J L GalziJ P Changeux
Jan 1, 1985·Annual Review of Cell Biology·R M Stroud, J Finer-Moore
Aug 1, 1986·Molecular and Cellular Biology·M Sudol, H Hanafusa
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P NefM Ballivet
Jun 1, 1983·Molecular and Cellular Biology·P C Cotton, J S Brugge
Feb 16, 1995·Nature·T Pawson
May 10, 1995·Annals of the New York Academy of Sciences·S L SwopeR L Huganir
Jun 1, 1995·The International Journal of Biochemistry & Cell Biology·P KefalasP M Brickell
Apr 1, 1995·Current Opinion in Cell Biology·T Erpel, S A Courtneidge
Aug 1, 1995·FEBS Letters·G Superti-Furga
Jul 1, 1994·Neuron·J SugiyamaZ W Hall
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·C M PleimanJ C Cambier
Feb 1, 1994·Current Opinion in Genetics & Development·J C Cambier, W A Jensen
Jan 28, 1994·Cell·A Weiss, D R Littman
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·G M Twamley-SteinS A Courtneidge
May 17, 1996·Cell·D J GlassG D Yancopoulos

❮ Previous
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Citations

Dec 19, 2001·Journal of Neurobiology·Lucia S BorgesMichael Ferns
Nov 19, 2002·Journal of Neurobiology·Steven J Burden
Oct 17, 1998·Biochemical Pharmacology·M W Salter
Sep 28, 2000·Trends in Neurosciences·C Heuss, U Gerber
Feb 17, 1998·Trends in Neurosciences·M A Ruegg, J L Bixby
Nov 21, 2001·Nature Reviews. Neuroscience·J R Sanes, J W Lichtman
Feb 25, 2006·Nature Neuroscience·Irwin B Levitan
Sep 22, 1999·European Journal of Biochemistry·W Hoch
Jul 21, 1998·Annals of the New York Academy of Sciences·J R SanesG D Yancopoulos
Apr 25, 2007·Molecular and Cellular Biology·Nina JonesTony Pawson
Sep 2, 2004·Molecular and Cellular Biology·Peggy MittaudChristian Fuhrer
Apr 15, 1999·Annual Review of Neuroscience·J R Sanes, J W Lichtman
Nov 3, 2011·PloS One·Hailong Luke Zhang, H Benjamin Peng
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·M Narisawa-SaitoH Nawa
Dec 6, 1997·Current Opinion in Genetics & Development·O deLapeyrière, C E Henderson
Sep 20, 2000·The Journal of Cell Biology·Z DaiH B Peng
May 1, 2007·Molecular and Cellular Neurosciences·Kristin BaerChristian Fuhrer
Nov 1, 2003·Annals of the New York Academy of Sciences·Alexandre MéjatLaurent Schaeffer
Aug 26, 2006·The EMBO Journal·Raffaella WillmannChristian Fuhrer
Jan 16, 2010·The European Journal of Neuroscience·Peter H ChipmanVictor F Rafuse
Nov 5, 2013·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Nadine KabbaniDavid J Adams
Mar 2, 2005·Molecular and Cellular Neurosciences·Raghavan MadhavanH Benjamin Peng
Aug 4, 1998·Current Opinion in Neurobiology·M Colledge, S C Froehner
Nov 2, 2011·Biochimica Et Biophysica Acta·Ana S Vallés, Francisco J Barrantes
Aug 22, 2016·Molecular Neurobiology·Neha SamiImtaiyaz Hassan
Dec 25, 2002·Journal of Neurobiology·S K Sharma, B G Wallace
Jul 31, 2007·Developmental Neurobiology·Xiaotao T ZhaoH Benjamin Peng
Oct 21, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Laure StrochlicJean Cartaud

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