PMID: 9444994Jan 28, 1998Paper

Functional interaction of the bovine papillomavirus E2 transactivation domain with TFIIB

Journal of Virology
J M YaoE J Androphy

Abstract

Induction of gene expression by the papillomavirus E2 protein requires its approximately 220-amino-acid amino-terminal transactivation domain (TAD) to interact with cellular factors that lead to formation of an activated RNA polymerase complex. These interaction partners have yet to be identified and characterized. The E2 protein localizes the transcription complex to the target promoter through its carboxy-terminal sequence-specific DNA binding domain. This domain has been reported to bind the basal transcription factors TATA-binding protein and TFIIB. We present evidence establishing a direct interaction between amino acids 74 to 134 of the E2 TAD and TFIIB. Within this region, the E2 point mutant N127Y was partially defective and W99C was completely defective for TFIIB binding in vitro, and these mutants displayed reduced or no transcriptional activity, respectively, upon transfection into C33A cells. Overexpression of TFIIB specifically restored transactivation by N127Y to close to wild-type levels, while W99C remained inactive. To further demonstrate the functional interaction of TFIIB with the wild-type E2 TAD, this region was fused to a bacterial DNA binding domain (LexA:E2:1-216). Upon transfection with increasing amoun...Continue Reading

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Citations

Apr 17, 1999·Molecular and Cellular Biology·D Mackey, B Sugden
Sep 17, 1999·The Journal of Biological Chemistry·K W VanceI M Morgan
Jul 13, 2007·Journal of Virology·Michal-Ruth SchweigerPeter M Howley
Nov 16, 2012·Journal of Virology·Edward J QuinlanElliot J Androphy
Jan 6, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Tipaya EkalaksanananChamsai Pientong
Apr 14, 2006·Journal of Virology·Michal-Ruth SchweigerPeter M Howley
Jun 14, 2000·Journal of Virology·Y C PengE J Androphy
Feb 29, 2008·Archives of Virology·Agnieszka K Olejnik-SchmidtAnna Goździcka-Józefiak
Jul 5, 2005·Journal of Virology·Maria G McPhillipsAlison A McBride
May 21, 2016·Chemical Biology & Drug Design·Worrapon KantangKuakarun Krusong
Sep 20, 2002·The Journal of Biological Chemistry·Samuel Y HouCheng-Ming Chiang
Jul 16, 2013·Virology·Alison A McBride
May 28, 2003·Biochemical and Biophysical Research Communications·Jiyun KimJoonho Choe

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