Functional interaction of transmembrane helices 3 and 6 in rhodopsin. Replacement of phenylalanine 261 by alanine causes reversion of phenotype of a glycine 121 replacement mutant.

The Journal of Biological Chemistry
M HanT P Sakmar

Abstract

Replacement of a highly conserved glycine residue on transmembrane (TM) helix 3 of bovine rhodopsin (Gly121) by amino acid residues with larger side chains causes a progressive blue-shift in the lambdamax value of the pigment, a decrease in thermal stability, and an increase in reactivity with hydroxylamine. In addition, mutation of Gly121 causes a relative reversal in the selectivity of opsin for 11-cis-retinal over all-trans-retinal. It was suggested that Gly121 plays an important role in defining the 11-cis-retinal binding pocket of rhodopsin (Han, M., Lin, S. W., Smith, S. O., and Sakmar, T. P. (1996) J. Biol. Chem. 271, 32330-32336). Here, we combined the mutant opsin G121L with second site replacements of four different amino acid residues on TM helix 6: Met257, Val258, Phe261, or Trp265. We show that the loss of function phenotypes of the G121L mutant described above can be partially reverted specifically by the mutation of Phe261, a residue highly conserved in all G protein-coupled receptors. For example, the double-replacement mutant G121L/F261A has spectral, chromophore-binding, and transducin-activating properties intermediate between those of G121L and rhodopsin. This rescue of the G121L defects did not occur with t...Continue Reading

References

Jan 1, 1992·DNA and Cell Biology·W C ProbstS C Sealfon
Oct 27, 1992·Biochemistry·E A ZhukovskyD D Oprian
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T P SakmarH G Khorana
Oct 5, 1990·Science·R R FrankeK P Hofmann
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T P SakmarH G Khorana
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R R BirgeK Nakanishi
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S S KarnikH G Khorana
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·J A JavitchA Karlin
Apr 22, 1993·Nature·G F SchertlerR Henderson
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·G F Schertler, P A Hargrave
Dec 13, 1996·The Journal of Biological Chemistry·M HanT P Sakmar

❮ Previous
Next ❯

Citations

May 7, 2003·Progress in Biophysics and Molecular Biology·Arthur D Conigrave, Alison H Franks
Apr 17, 1999·Journal of Photochemistry and Photobiology. B, Biology·I M Pepe
Oct 6, 2001·Progress in Retinal and Eye Research·I M Pepe
Nov 9, 2012·The Journal of Biological Chemistry·Louise Valentin-HansenThue W Schwartz
Jan 30, 2009·The Journal of Biological Chemistry·Shivani AhujaSteven O Smith
Oct 13, 2006·Annual Review of Pharmacology and Toxicology·Martine J SmitRob Leurs
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·M HanS O Smith
Jul 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Elsa C Y YanRichard A Mathies
Feb 15, 2002·Human Fertility : Journal of the British Fertility Society·Susan Benoff
May 27, 2004·Proteins·Paul R GouldsonChristopher A Reynolds
Mar 17, 2010·Biochemical Pharmacology·Marie Mi BondeJakob Lerche Hansen
Mar 24, 2015·Journal of Magnetic Resonance·Naoki KimataSteven O Smith
Dec 26, 2006·Biochimica Et Biophysica Acta·Brian K Kobilka
Aug 31, 1999·Biophysical Journal·J M Sullivan, P Shukla
Jan 25, 2002·Biophysical Journal·Nicole LehmannKarim Fahmy
May 4, 2002·Annual Review of Biophysics and Biomolecular Structure·Thomas P SakmarElias S Awad
Jan 25, 2002·Annual Review of Pharmacology and Toxicology·Lei Shi, Jonathan A Javitch
Jun 13, 1997·Journal of Molecular Biology·T ShiehS O Smith
Sep 12, 1997·The Journal of Biological Chemistry·M HanS O Smith
Jun 15, 2004·The Journal of Biological Chemistry·Aleksandar StojanovicJohn Hwa
Jun 2, 2006·Molecular Systems Biology·Antonio del SolRuth Nussinov
May 26, 2005·Pharmacological Reviews·Dianne M Perez, Sadashiva S Karnik
Dec 4, 2019·Nature Chemical Biology·Kuang-Yui Michael ChenPatrick Barth
Jul 11, 1998·The Journal of Biological Chemistry·U Gether, B K Kobilka
Sep 3, 1998·The Journal of Biological Chemistry·F DeLangeK J Rothschild
Aug 15, 2001·The Journal of Biological Chemistry·R Vogel, F Siebert
Dec 5, 2002·The Journal of Peptide Research : Official Journal of the American Peptide Society·R SinghF Guarnieri
Jan 15, 2000·The Journal of Biological Chemistry·E P MarinT P Sakmar
Dec 13, 1996·The Journal of Biological Chemistry·M HanT P Sakmar
Oct 3, 2001·Physiological Reviews·S T MenonT P Sakmar

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.