Functional interactions of red cells sensitized by IgG1 and IgG3 human monoclonal anti-D with enzyme-modified human monocytes and FcR-bearing cell lines

Molecular Immunology
B M Kumpel, A G Hadley

Abstract

Human monoclonal IgG1 and IgG3 antibodies specific for the Rh antigen D (anti-D) were tested for their ability to promote the binding of D-positive red cells to peripheral blood monocytes and Fc receptor (FcR)-bearing cell lines (U937, K562 and Daudi). Monocyte-mediated antibody-dependent cell-mediated cytotoxicity and metabolic (chemiluminescent) responses were also determined. By comparing the activity of different cell lines in rosette assays, and by using murine myeloma IgG2a and IgG1 to block FcRI and FcRII respectively, these functional interactions of sensitized red cells (E-IgG1 and E-IgG3) with monocytes or cell lines were shown to be mediated predominantly and perhaps solely by FcRI. E-IgG3 bound to human monocytes and cell lines to a greater extent than E-IgG1. Rosette formation by E-IgG3 was relatively less susceptible to inhibition by fluid-phase murine IgG2a than was rosette formation by E-IgG1. These findings may be due to the long hinge region of IgG3 which enables it to bridge the gap between two negatively charged cells more efficiently than IgG1. Consistent with this hypothesis was the greatly increased rosette formation achieved by treating monocytes or U937 cells with neuraminidase or bromelain, procedures ...Continue Reading

References

Jan 1, 1978·Vox Sanguinis·C J van OssR K Cunningham
Aug 1, 1985·The Journal of Clinical Investigation·D J NelsonR C Bone
Jan 1, 1989·Clinical and Laboratory Haematology·A G HadleyB Zupanska
Sep 1, 1988·Transfusion Medicine Reviews·J M Bowman
Dec 1, 1987·Scandinavian Journal of Immunology·J G Van de WinkelR A Koene
Jul 1, 1988·Cellular Immunology·M S ChiofaloM F La Via
Sep 1, 1988·European Journal of Immunology·R J KlaassenA E Von dem Borne
Sep 1, 1986·Immunology Today·C L Anderson, R J Loone

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Citations

Aug 24, 1999·European Journal of Immunology·K L ArmourL M Williamson
Apr 1, 1993·Molecular Immunology·J D PoundR Jefferis
Feb 15, 1992·Immunology Letters·B M Kumpel, T Bakács
Oct 1, 1995·Transfusion Medicine Reviews·A G Hadley
Sep 10, 2002·Transplant Immunology·Belinda M Kumpel
Mar 14, 2002·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·Belinda M Kumpel
Mar 1, 1992·Transfusion Medicine·C P Engelfriet
Aug 1, 1990·Journal of Immunogenetics·B M Kumpel
Feb 17, 2007·Transfusion·Leif Kofoed NielsenMorten Hanefeld Dziegiel
Jun 29, 2005·British Journal of Haematology·Annet van Royen-KerkhofJan G J van de Winkel
Jun 1, 1993·Baillière's Clinical Haematology·A G Hadley, B M Kumpel
Oct 1, 1995·Transfusion Medicine Reviews·A Fletcher, A Thomson
May 27, 2006·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Leif Kofoed NielsenMorten Hanefeld Dziegiel
Mar 9, 2011·Journal of Pharmaceutical and Biomedical Analysis·Keita Yamada, Kazuaki Kakehi
Jul 25, 2000·Journal of Clinical Laboratory Analysis·B K Flesch, J Neppert
Jan 1, 1995·Journal of Pineal Research·R J ReiterD Acuña-Castroviejo

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