PMID: 4899876Jul 1, 1969Paper

Functional interdependence of ribosomal components of Escherichia coli

Proceedings of the National Academy of Sciences of the United States of America
D Apirion, D Schlessinger

Abstract

Ribosomes are made up of parts that interact so strongly that a mutation in one of them can mask the effect of mutation in another. For example, when a mutation to neomycin resistance, which is a ribosome mutation, is introduced into cells carrying a ribosome mutation to spectinomycin resistance, some of the doubly mutant strains were phenotypically sensitive to spectinomycin, even though the mutation to spectinomycin resistance is still intact and recoverable in appropriate crosses. The neomycin mutant alleles that cause masking were shown by genetic tests to be in an identified locus that affects ribosomes. Protein synthesis in cell-free extracts of a double mutant strain was as sensitive to the action of spectinomycin as was the extract of the doubly sensitive parental strain. Thus, the masking effect of neomycin mutations on the spectinomycin mutation is exerted at the level of the ribosomes.We conclude that the genetic analysis of an organelle like the ribosome is likely to be severely complicated by pleiotropic effects and by interactions among its component parts and that the ribosomal binding sites for spectinomycin and neomycin are partially interdependent. These results suggest that the function of the ribosome requir...Continue Reading

References

Mar 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·S L PhillipsD Apirion
Sep 3, 1965·Science·J DaviesB D Davis
Jun 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·M R Capecchi

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Citations

Apr 25, 1978·Molecular & General Genetics : MGG·S H ThorbjarnardóttirG Eggertsson
Jan 1, 1974·Molecular & General Genetics : MGG·L A SaltzmanD Apirion
Jan 1, 1974·Molecular & General Genetics : MGG·D Pardo, R Rosset
Nov 29, 1977·Molecular & General Genetics : MGG·J N Davidson, L Bogorad
May 9, 1973·Molecular & General Genetics : MGG·B Tyler, J L Ingraham
Jan 1, 1983·Molecular & General Genetics : MGG·P PhoenixL Brakier-Gingras
Jan 1, 1982·Molecular & General Genetics : MGG·E S BogdanovaZ G Shmerling
Jan 1, 1974·Molecular & General Genetics : MGG·D Apirion, L Saltzman
Nov 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·C L BunnA W Linnane
May 1, 1976·Antimicrobial Agents and Chemotherapy·T YamadaK Suga
Jul 28, 1970·Journal of Molecular Biology·N Harford, N Sueoka
Mar 14, 1971·Journal of Molecular Biology·P S Sypherd
Jan 1, 1971·Molecular & General Genetics : MGG·T S IlyinaR B Khesin
Sep 1, 1970·Bacteriological Reviews·M Nomura
Jun 1, 1975·Journal of Bacteriology·P E Berg, S S Kang

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