Functional investigation of the chromosomal ccdAB and hipAB operon in Escherichia coli Nissle 1917.

Applied Microbiology and Biotechnology
Jun XuXinle Liang

Abstract

Toxin-antitoxin systems (TASs) have attracted much attention due to their important physiological functions. These small genetic factors have been widely studied mostly in commensal Escherichia coli strains, whereas the role of TASs in the probiotic E. coli Nissle 1917 (EcN) is still elusive. Here, the physiological role of chromosomally encoded type II TASs in EcN was examined. We showed that gene pair ECOLIN_00240-ECOLIN_00245 and ECOLIN_08365-ECOLIN_08370 were two functional TASs encoding CcdAB and HipAB, respectively. The homologs of CcdAB and HipAB were more conserved in E. coli species belonging to pathogenic groups, suggesting their important roles in EcN. CRISPRi-mediated repression of ccdAB and hipAB significantly reduced the biofilm formation of EcN in the stationary phase. Moreover, ccdAB and hipAB were shown to be responsible for the persister formation in EcN. Biofilm and persister formation of EcN controlled by the ccdAB and hipAB were associated with the expression of genes involved in DNA synthesis, SOS response, and stringent response. Besides, CRISPRi was proposed to be an efficient tool in annotating multiple TASs simultaneously. Collectively, our results advance knowledge and understanding of the role of TAS...Continue Reading

References

Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T Ogura, S Hiraga
Jun 23, 2001·The Journal of Antimicrobial Chemotherapy·J M Andrews
Sep 13, 2002·Canadian Journal of Microbiology·Grant J Balzer, Robert J C McLean
Nov 28, 2007·Journal of Bacteriology·Tim DurfeeDing Jun Jin
May 7, 2008·Infection and Immunity·Richard R KulesusMatthew A Mulvey
Jan 1, 1991·International Journal of Antimicrobial Agents·H H Gadebusch, D L Shungu
Jan 30, 2010·Journal of Medical Microbiology·Viktoria HancockPer Klemm
Jan 24, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Roy R Chaudhuri, Ian R Henderson
Mar 7, 2012·Antimicrobial Agents and Chemotherapy·Martina BielaszewskaHelge Karch
Jul 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Arti TripathiRaghavan Varadarajan
Sep 25, 2012·Applied and Environmental Microbiology·Jessica R SheldonKam Tin Leung
Jan 19, 2013·Microbiology·Junqiao ZhaoQingsheng Qi
Oct 8, 2013·Molecular Cell·Elsa GermainKenn Gerdes
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Dec 10, 2013·Applied Biochemistry and Biotechnology·Padma AmbalamAsa Ljungh
Dec 27, 2013·Nucleic Acids Research·Hailong WangA Francis Stewart
Mar 13, 2014·Microbial Cell Factories·Bjarte Aarmo LundGro Elin Kjæreng Bjerga
Nov 8, 2014·Microbiology·Krzysztof FiedorukKatarzyna Leszczynska
Dec 17, 2015·FEMS Microbiology Letters·Bhaskar Chandra Mohan Ramisetty, Ramachandran Sarojini Santhosh
Mar 19, 2016·Nature Chemical Biology·Rebecca Page, Wolfgang Peti
Jun 1, 2016·Frontiers in Microbiology·Paula M M MartinsAlessandra A de Souza
Jul 20, 2016·Gastroenterology Research and Practice·Artur DembińskiPeter Christopher Konturek
Sep 19, 2016·Applied Microbiology and Biotechnology·Bingming OuGuoqiang Zhu
Mar 12, 2017·Applied Microbiology and Biotechnology·Archana Somabhai ChaudhariG Naresh Kumar
Mar 24, 2017·FEMS Microbiology Reviews·Bram Van den BerghJan Michiels
Apr 28, 2017·FEMS Microbiology Reviews·Qiu E Yang, Timothy R Walsh
Jul 5, 2017·Journal of Bacteriology·Kritika GuptaRaghavan Varadarajan

❮ Previous
Next ❯

Methods Mentioned

BETA
genetic
gene knock-in
PCR
RNA Extraction
gene knockdown

Software Mentioned

TA finder
CDD
BLAST
Origin
MEGA6

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Related Papers

Ophthalmology
John D Gottsch, Oliver D Schein
Current Opinion in Ophthalmology
Karny Shouchane-BlumIrit Bahar
Journal of Cataract and Refractive Surgery
Nick Mamalis
© 2022 Meta ULC. All rights reserved