Functional involvement of serum response factor in the transcriptional regulation of caldesmon gene

Biochemical and Biophysical Research Communications
T MomiyamaK Sobue

Abstract

A 22-bp fragment including the CArG element (CArG1) is essential for the transcription of the caldesmon gene. In this study, we investigated the effects of serum response factor (SRF) on the functional regulation of caldesmon promoter in smooth muscle cells. Gel supershift assay revealed that SRF was one component of the CArG1-protein complex. Dominant-negative mutants of SRF suppressed the promoter activity of caldesmon, whereas wild-type SRF overcame this suppression. These results suggest that SRF functions as a core activating factor of the caldesmon promoter. Furthermore, fractionation of smooth muscle cells' nuclear extracts using DNA affinity paramagnetic particles suggests that SRF transactivates the caldesmon promoter in concert with additional factors in the flow-through fraction recruited to the CArG element.

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Citations

Oct 13, 2006·Molecular and Cellular Biology·Ken'ichiro HayashiKenji Sobue
Nov 16, 2010·European Journal of Cell Biology·Pierre MorinMichael F Olson
Feb 26, 2011·Cell Adhesion & Migration·Taira Mayanagi, Kenji Sobue
Oct 20, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D LiM Blumenberg
Mar 29, 2000·The Journal of Biological Chemistry·J M MianoR P Misra
Sep 21, 2013·Journal of Cell Science·Sang-Min JangSangmyung Rhee

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