Functional magnetic resonance mapping of intracerebroventricular infusion of a neuroactive peptide in the anaesthetised rat

Journal of Neuroscience Methods
Alessandro GozziAngelo Bifone

Abstract

Pharmacological magnetic resonance imaging (phMRI) methods map the cerebral haemodynamic response to challenge with psychotropic agents as a surrogate for drug-induced changes in brain activity. However, many neuroactive compounds present low blood-brain barrier penetration and thus systemic administration may result in insufficient brain concentration. Intracerebroventricular (ICV) administration has been long used as an effective way of bypassing the blood-brain barrier in studies with non-brain-penetrant compounds, such as neuropeptides. In order to extend the range of pharmacological substances accessible to phMRI, we have developed methods to map relative cerebral blood volume (rCBV) changes induced by in situ ICV administration of neuroactive agents in the anaesthetised rat. We have applied this method to study for the first time the phMRI response to central administration of a neuropeptide, the metabolically stable and potent NK1 receptor agonist GR-73632. ICV administration of 4.2 pmol of GR-73632 produced a rapid onset and sustained rCBV increase in several brain structures, such as the amygdala, the caudate putamen and the cortex. These results demonstrate the feasibility of phMRI as a tool to study the functional co...Continue Reading

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Citations

Mar 22, 2005·Psychopharmacology·C A StewardY B Shah
Nov 18, 2005·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Alessandro GozziAngelo Bifone
Aug 1, 2006·Expert Opinion on Drug Discovery·Gerard B FoxYanping Luo
Aug 29, 2006·Journal of Neuroscience Methods·Adam J SchwarzAngelo Bifone
May 23, 2006·Lancet Neurology·Helene Benveniste, Stephen J Blackband
Jul 20, 2005·Pharmaceutical Research·Conrad E JohansonAndrew Baird
Apr 11, 2006·Nature Reviews. Drug Discovery·David BorsookRichard Hargreaves

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