Functional, Metabolic and Morphologic Results of Ex Vivo Donor Lung Perfusion with a Perfluorocarbon-Based Oxygen Carrier Nanoemulsion in a Large Animal Transplantation Model.

Cells
Ilhan InciDonat R Spahn

Abstract

Ex vivo lung perfusion (EVLP) is a technology that allows the re-evaluation of questionable donor lung before implantation and it has the potential to repair injured donor lungs that are otherwise unsuitable for transplantation. We hypothesized that perfluorocarbon-based oxygen carrier, a novel reconditioning strategy instilled during EVLP would improve graft function. We utilized perfluorocarbon-based oxygen carrier (PFCOC) during EVLP to recondition and improve lung graft function in a pig model of EVLP and lung transplantation. Lungs were retrieved and stored for 24 h at 4 °C. EVLP was done for 6 h with or without PFCOC. In the transplantation groups, left lung transplantation was done after EVLP with or without PFCOC. Allograft function was assessed by means of pulmonary gas exchange, lung mechanics and vascular pressures, histology and transmission electron microscopy (TEM). In the EVLP only groups, physiological and biochemical markers during the 6-h perfusion period were comparable. However, perfusate lactate potassium levels were lower and ATP levels were higher in the PFCOC group. Radiologic assessment revealed significantly more lung infiltrates in the controls than in the PFCOC group (p = 0.04). In transplantation gr...Continue Reading

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Citations

Jan 1, 2021·International Journal of Molecular Sciences·Silke B BodewesRobert J Porte
Jun 14, 2021·Advances in Colloid and Interface Science·Marie Pierre Krafft, Jean G Riess
Aug 28, 2021·International Journal of Molecular Sciences·Njikem Asong-FontemRené Adam

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Methods Mentioned

BETA
Bronchoalveolar Lavage
protein assay
fluorescence spectroscopy
light microscopy

Software Mentioned

GraphPad
PRISM
Gatan Microscopical Suite
Excel

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