PMID: 11921395Mar 29, 2002Paper

Functional mimicry of a discontinuous antigenic site by a designed synthetic peptide

Chembiochem : a European Journal of Chemical Biology
Judit VillénDavid Andreu

Abstract

Functional reproduction of the discontinuous antigenic site D of foot-and-mouth disease virus (FMDV) has been achieved by means of synthetic peptide constructions that integrate each of the three protein loops that define the antigenic site into a single molecule. The site D mimics were designed on the basis of the X-ray structure of FMDV type C-S8c1 with the aid of molecular dynamics, so that the five residues assumed to be involved in antigenic recognition are located on the same face of the molecule, exposed to solvent and defining a set of native-like distances and angles. The designed site D mimics are disulfide-linked heterodimers that consist of a larger unit containing VP2(71-84), followed by a polyproline module and by VP3(52-62), and a smaller unit corresponding to VP1(188-194) (VP=viral protein). Guinea pig antisera to the peptides recognized the viral particle and competed with site D-specific monoclonal antibodies, while inoculation with a simple (not covalently joined to one another) admixture of the three VP1-VP3 sequences yielded no detectable virus-specific serum conversion. Similar results have been reproduced in two bovines. Antisera to the peptides also moderately neutralize FMDV in cell cultures and partial...Continue Reading

Citations

Oct 22, 2008·Current Opinion in Chemical Biology·Jutta Eichler
May 24, 2005·Analytical Biochemistry·Christina N Carrigan, Barbara Imperiali
Dec 4, 2014·Chembiochem : a European Journal of Chemical Biology·Linde E J SmeenkPeter Timmerman
Aug 26, 2006·Chemistry & Biology·Judit VillénDavid Andreu
Dec 13, 2007·Journal of Molecular Recognition : JMR·Peter TimmermanRob H Meloen
Aug 9, 2002·The Journal of Biological Chemistry·Kalanethee Paul-PletzerJerome Parness
May 4, 2021·Journal of Immunology Research·Masayoshi AosasaMujo Kim
Jan 20, 2006·Journal of Medicinal Chemistry·Miquel Vila-PerellóDavid Andreu
Feb 16, 2012·Organic Letters·Linde E J SmeenkPeter Timmerman

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