Functional optoacoustic neuro-tomography for scalable whole-brain monitoring of calcium indicators

Light, Science & Applications
X Luís Deán-BenDaniel Razansky

Abstract

Non-invasive observation of spatiotemporal activity of large neural populations distributed over entire brains is a longstanding goal of neuroscience. We developed a volumetric multispectral optoacoustic tomography platform for imaging neural activation deep in scattering brains. It can record 100 volumetric frames per second across scalable fields of view ranging between 50 and 1000 mm3 with respective spatial resolution of 35-200 μm. Experiments performed in immobilized and freely swimming larvae and in adult zebrafish brains expressing the genetically encoded calcium indicator GCaMP5G demonstrate, for the first time, the fundamental ability to directly track neural dynamics using optoacoustics while overcoming the longstanding penetration barrier of optical imaging in scattering brains. The newly developed platform thus offers unprecedented capabilities for functional whole-brain observations of fast calcium dynamics; in combination with optoacoustics' well-established capacity for resolving vascular hemodynamics, it could open new vistas in the study of neural activity and neurovascular coupling in health and disease.

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Citations

Jan 25, 2018·Scientific Reports·Héctor EstradaDaniel Razansky
Feb 1, 2019·Journal of Biophotonics·Benedict Mc LarneyDaniel Razansky
Sep 12, 2017·Nature Methods·Dal Hyung KimDrew N Robson
Feb 24, 2019·Nature Communications·Ali BarandovAlan Jasanoff
Feb 23, 2020·Journal of Neural Engineering·Jeeun KangDean F Wong
Oct 4, 2019·Nature Communications·Cody J Greer, Timothy E Holy
Nov 16, 2017·Optics Letters·Zhenyue ChenDaniel Razansky
Sep 22, 2018·Scientific Reports·Hsiao-Chun Amy LinDaniel Razansky
Feb 8, 2019·Light, Science & Applications·Elena MerčepDaniel Razansky
Apr 18, 2019·Nature Biomedical Engineering·Sven GottschalkDaniel Razansky
May 10, 2019·Frontiers in Neuroscience·Sven GottschalkDaniel Razansky
Jul 26, 2019·Physics in Medicine and Biology·Xosé Luís Deán-Ben, Daniel Razansky
Feb 19, 2017·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Assaf A Gilad, Mikhail G Shapiro
Mar 2, 2017·Optics Letters·X Luís Deán-BenDaniel Razansky
Aug 2, 2017·Scientific Reports·X Luís Deán-BenDaniel Razansky
Apr 8, 2017·Chemical Communications : Chem Comm·Vipul GujratiVasilis Ntziachristos
Jun 2, 2017·Scientific Reports·Bin RaoLihong V Wang
May 11, 2019·Nature Biomedical Engineering·Alessio Andreoni, Lin Tian
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Jan 1, 2018·Optica·Ali ÖzbekDaniel Razansky
Mar 10, 2017·Chemical Society Reviews·X L Deán-BenD Razansky
Mar 7, 2019·Light, Science & Applications·X Luís Dean-Ben, Daniel Razansky
Feb 3, 2021·Nature Materials·Arash FarhadiMikhail G Shapiro
Feb 18, 2021·European Journal of Nuclear Medicine and Molecular Imaging·Daniel RazanskyRuiqing Ni
Oct 16, 2020·Neuron·Claire RabutMikhail G Shapiro
Apr 10, 2018·Current Opinion in Chemical Biology·Junjie Yao, Lihong V Wang
May 15, 2021·Experimental Dermatology·Xosé Luís Deán-Ben, Daniel Razansky
Jun 2, 2021·Nature Biomedical Engineering·Shuai NaLihong V Wang
Jul 6, 2021·Frontiers in Neuroscience·Silviu-Vasile Bodea, Gil Gregor Westmeyer
May 13, 2020·Journal of the American Chemical Society·Tomas FialaDalibor Sames
Jan 22, 2019·ACS Sensors·Antonella LauriGil G Westmeyer
Aug 31, 2021·Biomedical Optics Express·Shuai Na, Lihong V Wang
Nov 5, 2021·Proceedings of the National Academy of Sciences of the United States of America·Çağla ÖzsoyDaniel Razansky

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Methods Mentioned

BETA
transgenic
light scattering
optical diffraction
light-scattering

Software Mentioned

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