Functional organization of the GluR1 glutamate receptor promoter

The Journal of Biological Chemistry
Karin Borges, Raymond Dingledine

Abstract

The GluR1 glutamate receptor subunit is expressed in most brain areas and plays a major role in excitatory synaptic transmission. We cloned and sequenced 5 kilobase pairs of the rat GluR1 promoter and identified multiple transcriptional start sites between -295 and -202 (relative to the first ATG). Similar to other glutamate receptor subunit promoters, the GluR1 promoter lacks TATA and CAAT elements in that region but binds Sp1 proteins at two sites. Promoter activity of GluR1 fragments cloned into pGL3 was assessed by immunocytochemistry and by measuring luciferase activity after transfection into primary cultures of rat cortical neurons and glia. GluR1 promoter activity was stronger in neurons, with neuronal specificity appearing to reside mainly within the neuronal expression-enhancing regions, -1395 to -743 and -253 to -48. The latter region contains 4 sites that bound recombinant cAMP-response element-binding proteins and a glial silencing region between -253 and -202. In both neurons and glia, promoter activity was increased by a 64-base pair GA repeat upstream of the initiation sites and reduced by a 57-base pair region that contained an N box. In contrast to the GluR2 promoter the regulatory regions are mainly located o...Continue Reading

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Citations

Jun 12, 2013·Nature Neuroscience·Erica KorbSteven Finkbeiner
Sep 1, 2012·Human Gene Therapy Methods·Aurélie DelzorNicole Déglon
Aug 19, 2010·Pharmacological Reviews·Stephen F TraynelisRay Dingledine
May 18, 2007·Annual Review of Cell and Developmental Biology·Jason D Shepherd, Richard L Huganir
Jun 1, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Vincent VialouEric J Nestler
Aug 13, 2003·Journal of Neurochemistry·Karin BorgesRaymond Dingledine
Nov 24, 2012·Journal of Neurochemistry·Shunhui WeiWeiping Han
Oct 15, 2003·Neuron·David S Bredt, Roger A Nicoll
Aug 12, 2017·International Journal of Molecular Sciences·Keishi IshidaYaichiro Kotake
Sep 24, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Takahiro NakayamaKimio Akagawa
Apr 14, 2007·The Journal of Biological Chemistry·Luca CrepaldiFrancesco Ferraguti
Aug 2, 2014·Behavioural Pharmacology·Elena H Chartoff, William A Carlezon
Feb 15, 2008·Science Signaling·Matt C Boersma, Mollie K Meffert

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