Functional overexpression of wild-type p53 correlates with alveolar cell differentiation in the developing human lung

The Anatomical Record
M TebarA A ten Have-Opbroek

Abstract

At 15 weeks after conception (a.c.), the human pulmonary acinus is lined by distal low-columnar and more proximal cuboidal cells that are successive stages in alveolar type II cell differentiation (pseudoglandular period of lung development). From 16 weeks a.c. onward, there are also 'flatter' cells that are intermediate stages in the differentiation of cuboidal type II cells into squamous type I cells (canalicular period). We investigated the role of wild-type p53 protein and the proliferation marker Ki-67 in the differentiation of type II and type I cells in these two periods. Serial sections from fetal lungs (n = 30) were immunoincubated with antibodies against p53 and Ki-67. The presence of prospective type II and type I cells was confirmed using immunohistochemistry for surfactant protein SP-A as a differentiation marker and light and electron microscopy. The p53 and Ki-67 positive nuclei were quantified per alveolar cell phenotype (i.e., low-columnar; cuboidal; flatter). The occurrence of cell apoptosis was studied using propidium iodide (PI) and 4',6'-diamino-2-phenylindol dihydrochloride (DAPI) staining. The combined increase in p53 expression and decrease in Ki-67 expression during alveolar epithelial cell differentiat...Continue Reading

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Citations

Dec 24, 2005·Anatomy and Embryology·David E KlingJay J Schnitzer
Apr 27, 2013·BMC Developmental Biology·Sana MujahidHeber C Nielsen
Jun 2, 2007·BMC Genomics·Christopher BrynczkaB Alex Merrick
Dec 16, 2010·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Xiaochen WangJun Zhao
Feb 28, 2015·Acta histochemica·Daniela KraljevicVioleta Soljic
Apr 13, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·E A O'BrienR H Lane

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