Functional pathway analysis in acute myeloid leukemia using single cell network profiling assay: effect of specimen source (bone marrow or peripheral blood) on assay readouts.

Cytometry. Part B, Clinical Cytometry
Alessandra CesanoElisabeth Paietta

Abstract

Single cell network profiling (SCNP) is used to simultaneously measure the effects of modulators on signaling networks at the single cell level. SCNP-based biomarker assays predictive of response to induction therapy and relapse risk in acute myeloid leukemia (AML) patients are being developed. Such assays have typically used bone marrow (BM) as the sample source of blasts. Because circulating peripheral blasts are detectable in ∼65% of AML patients and peripheral blood (PB) sampling is less invasive than BM sampling, this study was performed to assess the effect of sample source on AML blasts signaling as measured in SCNP assay. SCNP using multiparametric flow cytometry was used to evaluate the activation state of intracellular signaling molecules in leukemic blasts under basal conditions and after treatment with modulators in 46 pairs of BM mononuclear cells/PB mononuclear cells. The relationship between readouts of modulated intracellular proteins ("nodes") was measured using linear regression, Bland-Altman method, and Lin's concordance correlation coefficient. The majority (156/161) of signaling nodes show strong correlations between paired PB and BM samples independently from the statistical method used. Notable exceptions...Continue Reading

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Citations

Jul 12, 2012·Nature Biotechnology·Sean C Bendall, Garry P Nolan
Jun 8, 2012·Journal of Translational Medicine·Diane M LongoAlessandra Cesano
Jun 5, 2013·Journal of Translational Medicine·Paolo A AsciertoMagdalena Thurin
Aug 2, 2012·International Journal of Environmental Research and Public Health·Reuben ThomasLuoping Zhang
Jan 10, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Todd M CoveyAlessandra Cesano
Jun 26, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Alessandra Cesano, David Parkinson
Apr 29, 2015·Cytometry. Part B, Clinical Cytometry·Emilie CoppinJacques A Nunès
Feb 20, 2016·Expert Review of Molecular Diagnostics·Bjørn T GjertsenJörn Skavland
Aug 20, 2015·Journal for Immunotherapy of Cancer·Rachael E Hawtin, Alessandra Cesano
Apr 24, 2012·Leukemia Research·David B RosenAlessandra Cesano
Jul 13, 2019·Journal for Immunotherapy of Cancer·Silvia C FormentiSandra Demaria
Oct 10, 2013·Cytometry. Part B, Clinical Cytometry·Steven M KornblauAlessandra Cesano
Nov 30, 2016·Journal for Immunotherapy of Cancer·Giuseppe V MasucciMagdalena Thurin

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