Functional polymorphism of the CK2alpha intronless gene plays oncogenic roles in lung cancer.

PloS One
Ming-Szu HungLiang You

Abstract

Protein kinase CK2 is frequently up-regulated in human cancers, although the mechanism of CK2 activation in cancer remains unknown. In this study, we investigated the role of the CK2alpha intronless gene (CSNK2A1P, a presumed CK2alpha pseudogene) in the pathogenesis of human cancers. We found evidence of amplification and over-expression of the CSNK2A1P gene in non-small cell lung cancer and leukemia cell lines and 25% of the lung cancer tissues studied. The mRNA expression levels correlated with the copy numbers of the CSNK2A1P gene. We also identified a novel polymorphic variant (398T/C, I133T) of the CSNK2A1P gene and showed that the 398T allele is selectively amplified over the 398C allele in 101 non-small cell lung cancer tissue samples compared to those in 48 normal controls (p = 0.013<0.05). We show for the first time CSNK2A1P protein expression in transfected human embryonic kidney 293T and mouse embryonic fibroblast NIH-3T3 cell lines. Both alleles are transforming in these cell lines, and the 398T allele appears to be more transforming than the 398C allele. Moreover, the 398T allele degrades PML tumor suppressor protein more efficiently than the 398C allele and shows a relatively stronger binding to PML. Knockdown of ...Continue Reading

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Sep 20, 2012·Science Signaling·Laura Poliseno
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Jul 27, 2021·Frontiers in Genetics·Katia Aviña-PadillaMaribel Hernández-Rosales

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Methods Mentioned

BETA
transfection
Kinase
PML
co-immunoprecipitation
immunoprecipitation
surgical resection
PCR
reverse transcription-PCR
Assay

Software Mentioned

Primer Express Oligo Design
Allelic Discrimination Sequence Detection
SPSS

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