Functional profiling and crystal structures of isothiocyanate hydrolases found in gut-associated and plant pathogenic bacteria

Applied and Environmental Microbiology
Tijs J M van den BoschCornelia U Welte

Abstract

Isothiocyanates (ITCs) are produced by cruciferous plants to protect them against herbivores and infection by microbes. These compounds are of particular interest due to their antimicrobial and anticarcinogenic properties. The breakdown of ITCs in nature is catalyzed by isothiocyanate hydrolase (ITCase), a novel family within the metallo-β-lactamase-fold superfamily of proteins. saxA genes that code for ITCases are particularly widespread in insect- and plant-associated bacteria. Enzymatic characterization of seven phylogenetically related but distinct ITCases exhibited similar activities on six selected ITCs, suggesting that phylogenetic diversity does not determine the substrate specificity of ITCases. X-ray crystallography studies of two ITCases sharing 42% amino acid sequence identity revealed a highly conserved tertiary structure. Notable features of ITCase include a hydrophobic active site with two Zn2+ ions coordinating water/hydroxide, and a flexible cap that is implicated in substrate recognition that covers the active site. This report reveals the function and structure of the previously uncharacterized family of isothiocyanate hydrolases within the otherwise relatively well-studied superfamily of metallo-β-lactamases...Continue Reading

References

Mar 29, 2001·Journal of Bacteriology·J L SeffernickL P Wackett
Dec 26, 2001·Nucleic Acids Research·Raquel Tobes, Juan L Ramos
Jul 24, 2002·Nucleic Acids Research·Kazutaka KatohTakashi Miyata
Sep 28, 2002·Acta Crystallographica. Section D, Biological Crystallography·Thomas R Schneider, George M Sheldrick
Jul 21, 2004·Journal of Structural and Functional Genomics·Youngchang KimAndrzej Joachimiak
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·E Krissinel, K Henrick
Jun 30, 2005·Pest Management Science·John N Matthiessen, Mark A Shackleton
Dec 24, 2005·Journal of Synchrotron Radiation·Gerd RosenbaumAndrzej Joachimiak
May 4, 2006·Annual Review of Plant Biology·Barbara Ann Halkier, Jonathan Gershenzon
Jul 21, 2006·Acta Crystallographica. Section D, Biological Crystallography·Wladek MinorMaksymilian Chruszcz
Jan 1, 1997·Methods in Enzymology·A T Brünger
May 7, 2009·Acta Pharmacologica Sinica·Xiang WuKe Xu
Oct 16, 2009·Clinical and Vaccine Immunology : CVI·Giovanni MateraLeo A B Joosten
Dec 5, 2009·Molecular Nutrition & Food Research·Yuesheng Zhang
Jan 9, 2010·Acta Crystallographica. Section D, Biological Crystallography·Alexei Vagin, Alexei Teplyakov
May 12, 2010·Nucleic Acids Research·Liisa Holm, Päivi Rosenström
Feb 10, 2011·Standards in Genomic Sciences·Sabine GronowSusan Lucas
Oct 1, 2011·Nature Methods·Thomas Nordahl PetersenHenrik Nielsen
Mar 13, 2012·Phytochemistry·Niels Agerbirk, Carl Erik Olsen
Apr 17, 2012·Acta Crystallographica. Section D, Biological Crystallography·Pavel V AfoninePaul D Adams
Feb 1, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Youngchang KimAndrzej Joachimiak
Apr 4, 2013·BMC Genomics·Lex OvermarsChristof Francke
Jul 9, 2013·Scientia pharmaceutica· Faridoon, Nazar Ul Islam
Sep 10, 2013·Applied and Environmental Microbiology·Virginie DufourChristine Baysse
Oct 31, 2013·Molecular Nutrition & Food Research·Vijitra Luang-InJohn T Rossiter
Feb 11, 2014·Molecular Nutrition & Food Research·Parul GuptaSanjay K Srivastava
Nov 8, 2014·Microbiology·Virginie DufourChristine Baysse
Jul 26, 2015·Current Opinion in Structural Biology·P Andrew Karplus, Kay Diederichs
Jan 26, 2016·Trends in Biochemical Sciences·Ilaria PettinatiChristopher J Schofield
Feb 14, 2016·Applied and Environmental Microbiology·Cornelia U WelteMike S M Jetten

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Citations

Mar 17, 2020·Frontiers in Microbiology·Tijs J M van den Bosch, Cornelia U Welte
Dec 25, 2019·Molecular Plant Pathology·Tijs J M van den BoschCornelia U Welte

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