Functional properties of a Brazilian derived mouse embryonic stem cell line

Anais Da Academia Brasileira De Ciências
Leandro VairoAntonio C Campos De Carvalho

Abstract

Pluripotent mouse embryonic stem cells (mESC) are cell lines derived from the inner cell mass of blastocyst-stage early mammalian embryos. Since ion channel modulation has been reported to interfere with both growth and differentiation process in mouse and human ESC it is important to characterize the electrophysiological properties of newly generated mESC and compare them to other lines. In this work, we studied the intercellular communication by way of gap junctions in a Brazilian derived mESC (USP-1, generated by Dr. Lygia Pereira's group) and characterized its electrophysiological properties. We used immunofluorescence and RT-PCR to reveal the presence of connexin 43 (Cx43), pluripotency markers and ion channels. Using a co-culture of neonatal mouse cardiomyocytes with mESC, where the heart cells expressed the enhanced Green Fluorescent Protein, we performed dye injections to assess functional coupling between the two cell types observing dye diffusion. The patch-clamp study showed outward currents identified as two types of potassium currents, transient outward potassium current (Ito) and delayed rectifier outward potassium current (Iks), by use of specific drug blockage. Calcium or sodium currents in undifferentiated mESC...Continue Reading

References

Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D Solter, B B Knowles
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G R Martin
Nov 6, 1998·Science·J A ThomsonJ M Jones
Dec 11, 1999·Cells, Tissues, Organs·M PesceH R Schöler
May 16, 2002·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M A SukoyanL V Pereira
Oct 28, 2003·The Journal of Physiology·Jürgen F HeubachUrsula Ravens
Oct 31, 2003·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Marjan van KempenMarcel van der Heyden
Dec 19, 2003·Cell Communication & Adhesion·Goran Söhl, Klaus Willecke
Dec 23, 2003·Stem Cells·Mark RichardsAriff Bongso
Jan 28, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Melissa K CarpenterMahendra S Rao
Nov 13, 2004·Stem Cells·Raymond C B WongMartin F Pera
Dec 29, 2004·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Ardawan Julian RastanFriedrich Wilhelm Mohr
Oct 1, 2005·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Jens GarbadeFriedrich-Wilhelm Mohr
Apr 1, 2006·Stem Cells·James E HuettnerJohn W McDonald
Apr 18, 2006·Biochemical and Biophysical Research Communications·Raymond C B WongAlice Pébay
Jun 3, 2008·Europace : European Pacing, Arrhythmias, and Cardiac Electrophysiology : Journal of the Working Groups on Cardiac Pacing, Arrhythmias, and Cardiac Cellular Electrophysiology of the European Society of Cardiology·Emiliano Horacio MedeiAntonio C Campos de Carvalho
Oct 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Junji MorokumaMichael Levin
Dec 4, 2009·American Journal of Physiology. Cell Physiology·Peng JiangRonald A Li

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