PMID: 11334631May 4, 2001Paper

Functional properties of a truncated recombinant GIRK5 potassium channel

Biochimica Et Biophysica Acta
C SalvadorLaura I Escobar

Abstract

Xenopus laevis oocytes codify a G-protein-activated inward rectifier potassium channel (GIRK5 or Kir3.5). Coinjection of other GIRKs, the muscarinic m2 receptor, or Gbetagamma protein cRNAs is required to observe functional GIRKx-GIRK5 heteromultimers in oocytes. Studies with GIRK2 isoforms have shown that the size of the amino or carboxyl terminus plays a crucial role on giving functional K(+) channels. In this work we studied the properties of a GIRK5 with 25 amino acids deleted toward its amino-terminal domain. Injection of GIRK5-Delta25 cRNA alone displayed large basal and transient inward rectifying currents in oocytes. The instantaneous currents reached a stationary level after a long duration voltage pulse (10 s). For this relaxation, fast (tau(1)) and slow (tau(2)) time constants were estimated at different voltages. Recovery from inactivation followed a monoexponential function (tau=0.95+/-0.07 s). By contrast with other inward rectifier channels, blockade of GIRK5-Delta25 by extracellular Ba(2+) was voltage-independent (K(d)=102+/-2 microM), suggesting the presence of a Ba(2+) site at the external channel vestibule. To confirm this hypothesis, the Ba(2+) sensitivity of two charged mutants GIRK5-Delta25(N129E) and GIRK...Continue Reading

References

Apr 1, 1988·The Journal of General Physiology·R D Harvey, R E Ten Eick
Sep 1, 1996·Pflügers Archiv : European journal of physiology·C K BauerJ R Schwarz
Mar 1, 1996·The Journal of General Physiology·K W ChanD E Logothetis
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Jan 16, 1998·Cellular Signalling·N Dascal

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Citations

Nov 19, 2009·Histochemistry and Cell Biology·Rolando CarrisozaLaura I Escobar
Jan 22, 2003·Life Sciences·Carolina SalvadorLaura I Escobar
Jul 29, 2011·Kidney International·Rolando Carrisoza-GaytánLaura I Escobar
Dec 23, 2015·Journal of Cellular and Molecular Medicine·Wei ZhangZu-Yi Yuan
Nov 21, 2001·The Journal of Biological Chemistry·Carlos SaldañaLuis Vaca

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