PMID: 7521756Dec 1, 1993Paper

Functional properties of alternatively spliced forms of the Drosophila PS2 integrin alpha subunit

Cell Adhesion and Communication
M ZavortinkD L Brower

Abstract

The Drosophila alpha PS2 protein is encoded by two alternatively spliced transcripts. The respective alpha PS2 proteins differ by the presence of 25 amino acids in the alpha PS2(C) protein, not found in the alpha PS2(m8) subunit, in a region thought to be important for ligand binding. We examined the functional properties of Drosophila S2 cells transformed with genes expressing either of these proteins, in association with a beta PS subunit. Both PS2 integrins support cell spreading on vertebrate vitronectin or, to a lesser extent, on fibronectin. Interestingly, the PS2(C) form promotes spreading more efficiently on vitronectin than does the PS2(m8) form, with an opposite relative efficiency seen for fibronectin. Also, the two forms of PS2 show different requirements for divalent cations in order to mediate efficient cell spreading. These divalent cations are not required to maintain the association of alpha and beta subunits. Spreading of both cell types is similarly RGD sensitive, and both PS2 integrins appear to associate with the actin cytoskeleton. To our knowledge, this represents the first demonstration of functional differences in integrin subunits resulting from splicing variation to generate different extracellular, l...Continue Reading

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Citations

Jul 14, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A A de Melker, A Sonnenberg
Dec 18, 2012·European Biophysics Journal : EBJ·Dipak Mainali, Emily A Smith
Oct 2, 2003·Current Opinion in Cell Biology·Danny L Brower
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Apr 23, 2020·Journal of Cell Science·José C Pastor-Pareja
Aug 19, 2007·Molecular Biology of the Cell·Brian P JamesDanny L Brower

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