PMID: 15223783Jun 30, 2004Paper

Functional properties of hyalocytes under PDGF-rich conditions

Investigative Ophthalmology & Visual Science
Yoshihiro NodaTatsuro Ishibashi

Abstract

To investigate the functional properties and intracellular signaling of hyalocytes under platelet-derived growth factor (PDGF)-rich conditions. The hyalocytes were isolated from bovine eyes and identified by immunocytochemistry and electron microscope. The expression of PDGF receptor alpha/beta and its phosphorylation in response to PDGF-BB was analyzed by Western blot analysis. PDGF-BB-induced proliferation and migration were evaluated by thymidine uptake and Boyden's chemotaxis assay. The expression of the urokinase-type plasminogen activator (uPA) gene and the fibrinolytic activity were assessed by Northern blotting and fibrin zymography. An in vitro type I collagen gel contraction assay was performed to determine the effect of PDGF-BB on cellular contraction. The hyalocytes were immunocytochemically positive for S-100 and negative for glial fibrillary acidic protein (GFAP) and cytokeratin, as previously described. The electron microscope demonstrated that hyalocytes possess lysosome-like granules, mitochondria, and micropinocytotic vesicles in their cytoplasm. The hyalocytes expressed PDGF receptor alpha and beta, both of which were immediately phosphorylated in response to PDGF-BB. PDGF-BB also activated p85 PI3-kinase, p4...Continue Reading

Citations

May 8, 2008·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Florian SommerAchim Göpferich
Feb 16, 2010·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Yasuaki HataTatsuro Ishibashi
Mar 29, 2008·Japanese Journal of Ophthalmology·Yasuaki HataTatsuro Ishibashi
Oct 28, 2008·Proceedings of the National Academy of Sciences of the United States of America·Takeshi KitaTatsuro Ishibashi
May 24, 2007·Tissue Engineering·Florian SommerAchim Goepferich
Apr 17, 2007·Current Opinion in Ophthalmology·Taiichi Hikichi
Jul 24, 2010·Journal of Ophthalmology·Ryoichi AritaTatsuro Ishibashi
Aug 13, 2013·Journal of Biophysics·Chi-Li ChiuEnrico Gratton
Dec 17, 2008·Experimental Eye Research·Florian SommerAchim Göpferich
Aug 21, 2007·Experimental Eye Research·Koichi NishitsukaHidetoshi Yamashita
Mar 11, 2016·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·D Korkmaz, S Kum
Jul 2, 2009·Journal of Anatomy·Cristina LlombartJesús Ruberte
Nov 3, 2009·Acta Ophthalmologica·Yoshiko KashiwagiHidetoshi Yamashita
Feb 6, 2019·Vestnik oftalmologii·A A Suetov, E V Boiko
Apr 15, 2021·International Journal of Retina and Vitreous·Ismael ChehaibouRamin Tadayoni

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