Functional properties of LptA and LptD in Anabaena sp. PCC 7120

Biological Chemistry
Yi-Ching HsuehEnrico Schleiff

Abstract

Lipopolysaccharides (LPS) are central components of the outer membrane and consist of Lipid A, the core polysaccharide, and the O-antigen. The synthesis of LPS is initiated at the cytosolic face of the cytoplasmic membrane. The subsequent transport to and across the outer membrane involves multiple lipopolysaccharide transport (Lpt) proteins. Among those proteins, the periplasmic-localized LptA and the outer membrane-embedded LptD participate in the last steps of transfer and insertion of LPS into the outer membrane. While the process is described for proteobacterial model systems, not much is known about the machinery in cyanobacteria. We demonstrate that anaLptD (alr1278) of Anabaena sp. PCC 7120 is important for cell wall function and its pore domain shows a Lipid A sensitive cation-selective gating behavior. The N-terminal domain of anaLptD recognizes anaLptA (alr4067), but not ecLptA. Furthermore, anaLptA specifically interacts with the Lipid A from Anabaena sp. PCC 7120 only, while anaLptD binds to Lipid A isolated from Escherichia coli as well. Based on the comparative analysis of proteins from E. coli and Anabaena sp. we discuss the properties of the cyanobacterial Lpt system.

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Citations

Apr 22, 2021·Journal of Microbiology and Biotechnology·Hannah SchätzleEnrico Schleiff

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Methods Mentioned

BETA
PCR
chemical treatments
electron microscopy
transmission electron microscopy
size exclusion chromatography
affinity purification
dot blot
circular dichroism
nucleotide
GTPase

Software Mentioned

YASARA
HMMER
TMHMM
MAFFT

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