Functional properties of motoneurons derived from mouse embryonic stem cells

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
G B MilesR M Brownstone

Abstract

The capacity of embryonic stem (ES) cells to form functional motoneurons (MNs) and appropriate connections with muscle was investigated in vitro. ES cells were obtained from a transgenic mouse line in which the gene for enhanced green fluorescent protein (eGFP) is expressed under the control of the promotor of the MN specific homeobox gene Hb9. ES cells were exposed to retinoic acid (RA) and sonic hedgehog agonist (Hh-Ag1.3) to stimulate differentiation into MNs marked by expression of eGFP and the cholinergic transmitter synthetic enzyme choline acetyltransferase. Whole-cell patch-clamp recordings were made from eGFP-labeled cells to investigate the development of functional characteristics of MNs. In voltage-clamp mode, currents, including EPSCs, were recorded in response to exogenous applications of GABA, glycine, and glutamate. EGFP-labeled neurons also express voltage-activated ion channels including fast-inactivating Na(+) channels, delayed rectifier and I(A)-type K(+) channels, and Ca(2+) channels. Current-clamp recordings demonstrated that eGFP-positive neurons generate repetitive trains of action potentials and that l-type Ca(2+) channels mediate sustained depolarizations. When cocultured with a muscle cell line, clust...Continue Reading

Citations

Sep 10, 2011·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Genevieve Gowing, Clive N Svendsen
Aug 20, 2011·Cell Stem Cell·Esther Y SonKevin Eggan
Jul 21, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Mark E HesterBrian K Kaspar
Feb 3, 2005·Nature Biotechnology·Xue-Jun LiSu-Chun Zhang
Apr 17, 2007·Nature Neuroscience·Makiko NagaiSerge Przedborski
May 30, 2012·Human Molecular Genetics·Claudia FalliniWilfried Rossoll
Jul 7, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Patrick J Whelan
Jan 7, 2010·The Journal of Clinical Investigation·Olle Lindvall, Zaal Kokaia
Feb 6, 2014·Development·Carolina Barcellos MachadoIvo Lieberam
Jun 16, 2011·PloS One·Gabriel NistorHans S Keirstead
Jul 18, 2012·PloS One·Tomonori TakazawaAmy B Macdermott
Mar 23, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·M Austin JohnsonSu-Chun Zhang
Mar 9, 2013·Gene·Leonardo M R Ferreira, Mohammed A Mostajo-Radji
Aug 26, 2014·Assay and Drug Development Technologies·Jonathan J CherryJill Jarecki
Jun 20, 2012·Journal of Visualized Experiments : JoVE·Chia-Yen WuWenlan Wang
Jul 30, 2010·Cellular and Molecular Life Sciences : CMLS·M NizzardoS Corti
May 21, 2014·Biotechnology and Bioengineering·Dylan A McCreedyShelly E Sakiyama-Elbert
May 10, 2005·The Journal of Physiology·G B MilesR M Brownstone
Mar 1, 2012·Medicina clínica·Carme CostaXavier Montalban
Feb 2, 2012·Archives of Medical Research·Rodrigo López-González, Iván Velasco
Jun 29, 2011·Brain Research·Robert M Brownstone, Douglas G Stuart
Oct 26, 2010·Current Opinion in Neurobiology·Mirza Peljto, Hynek Wichterle
Nov 4, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J M HackettM Griffith
Apr 8, 2009·Experimental Neurology·Christina ChatziSanbing Shen
Mar 11, 2008·Trends in Neurosciences·Masatoshi Suzuki, Clive N Svendsen
Nov 21, 2007·Journal of Bioscience and Bioengineering·Ayako Kitazawa, Norio Shimizu
Jul 8, 2015·In Vitro Cellular & Developmental Biology. Animal·Zohreh BagherMohammad Taghi Joghataei

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