PMID: 2111240Mar 1, 1990Paper

Functional properties of proislet isografts in diabetic mice

Diabetes Research and Clinical Practice
C J SimeonovicJ D Wilson

Abstract

Fetal proislets (islet precursors) were transplanted to isogeneic diabetic CBA/H mice for analysis of their development and function post-transplant. Immunohistochemically, proislets contained scattered populations of insulin-, glucagon- and somatostatin-producing cells; following isotransplantation proislets developed into mature islet tissue with normal endocrine cell composition. Proislet isografts also developed normal endocrine function after transplantation to diabetic mice. Isografts of eight and four donor-equivalents of proislets reversed streptozotocin-induced diabetes by 30 +/- 7 and 74 +/- 26 days, respectively. Following stimulation with intragastric glucose (50 mg) mice carrying established isografts of four donor-equivalents of proislets exhibited normal blood glucose responses; those grafted with eight donor-equivalents of proislets showed decreased blood glucose levels. The beta cell mass of proislets continued to grow and differentiate post-transplant; established proislet grafts contained greater than 100-fold the total insulin content of the corresponding number of fetal pancreases from which the proislets were derived. The mean insulin content of eight and four donor-equivalent proislet grafts was 231% and ...Continue Reading

References

Apr 1, 1988·Transplantation·C J Simeonovic, K J Lafferty
Jan 1, 1985·Metabolism: Clinical and Experimental·J D WilsonS P Haynes
Apr 1, 1982·The Australian Journal of Experimental Biology and Medical Science·M AgostinoK J Lafferty
Aug 1, 1982·The Australian Journal of Experimental Biology and Medical Science·C J Simeonovic, K J Lafferty
Aug 1, 1982·The Australian Journal of Experimental Biology and Medical Science·C J Simeonovic, K J Lafferty

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