Functional properties of the anaerobic responsive element of the maize Adh1 gene

Plant Molecular Biology
M R OliveW J Peacock

Abstract

The functional properties of the anaerobic responsive element (ARE) of the maize Adh1 gene have been analysed using a transient expression assay in electroporated maize protoplasts. The ARE functions in both orientations although inversion of the ARE sequence relative to the TATA box element produces slightly weaker promoter activity under anaerobic conditions and elevated expression under aerobic conditions. Promoter activity under anaerobic conditions is proportional to the number of complete ARE sequences in the Adh1 promoter. The ARE contains two sub-regions and dimers of sub-region II are as efficient as the wild-type sequence in activating gene expression under anaerobic conditions. However, sub-region I dimers do not appear capable of inducing gene expression in response to anaerobic stress. We conclude that sub-region II is essential for anaerobic induction of gene expression. Reporter gene expression remains constant when the spacing between sub-regions of the ARE is increased up to at least 64 bp, but increased spacing of 136 bp or greater abolishes expression in both aerobic and anaerobic conditions, indicating that a close association of the two sub-regions is required both for anaerobic responsiveness and for maxim...Continue Reading

References

Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E LamN H Chua
Dec 1, 1987·Genes & Development·J CallisV Walbot
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C Chang, E M Meyerowitz
Feb 11, 1985·Nucleic Acids Research·E S DennisW J Peacock
Jan 1, 1985·Annual Review of Genetics·M Freeling, D C Bennett
Sep 1, 1986·Nature·M CombH M Goodman
Aug 20, 1988·Journal of Molecular Biology·E S DennisW J Peacock
May 5, 1987·Journal of Molecular Biology·D J LlewellynW J Peacock
Jul 1, 1980·Cell·M M SachsR Okimoto
Sep 1, 1993·The Plant Journal : for Cell and Molecular Biology·J G EllisW J Peacock
Apr 1, 1960·Archives of Biochemistry and Biophysics·R H HAGEMAN, D FLESHER
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J C WalkerW J Peacock
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·W L GerlachW J Peacock

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Citations

Jul 1, 1996·Plant Molecular Biology·M A HossainT K Hodges
Jan 24, 2006·Plant Molecular Biology·Elumalai Sivamani, Rongda Qu
Aug 11, 2000·Plant Science : an International Journal of Experimental Plant Biology·J WangJ H Oard
Jul 1, 1991·The Plant Cell·D Van der StraetenM Van Montagu
Mar 29, 2003·The Plant Journal : for Cell and Molecular Biology·Masumi Robertson
May 28, 2013·Protein Expression and Purification·Fangfang ChengYingwu Xu
Aug 12, 2000·Journal of Experimental Botany·E S DennisW J Peacock
May 1, 1991·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·D I LastE S Dennis
Jan 19, 2020·The New Phytologist·Travis A Lee, Julia Bailey-Serres
Feb 8, 2002·The Journal of Biological Chemistry·Peng-Wen ChenSu-May Yu
Jun 1, 1992·Evolution; International Journal of Organic Evolution·John W Y Chan, Ronald S Burton

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