Functional properties of the individual thioredoxin-like domains of protein disulfide isomerase

Biochemistry
N J Darby, T E Creighton

Abstract

The two thioredoxin-like domains of human protein disulfide isomerase (PDI) have been produced in bacteria as individual soluble, folded protein molecules, and their functional properties have been compared to those of intact PDI. The two individual domains were very similar in their functional properties, and there were no indications of synergy between them, so it is unlikely that they have intrinsically different functions in PDI. Both domains efficiently introduced disulfide bonds into unfolded model proteins and peptides but were less efficient than PDI with folded substrate protein molecules. Relative to PDI, neither domain had substantial activity in catalyzing disulfide bond isomerization. This pattern of activities is very similar to that of the bacterial catalyst DsbA and probably reflects similarities in the catalytic mechanisms of these proteins. The differences in activity between PDI and its thioredoxin-like domains suggest that other features of the PDI molecule are also required for its complete range of thiol-disulfide exchange activities.

Citations

Dec 1, 1995·Protein Science : a Publication of the Protein Society·J KemminkT E Creighton
May 18, 2007·Extremophiles : Life Under Extreme Conditions·Rudolf Ladenstein, Bin Ren
May 26, 2004·Biochimica Et Biophysica Acta·Bonney Wilkinson, Hiram F Gilbert
Sep 27, 2000·Biochimica Et Biophysica Acta·X X SunC C Wang
May 7, 2003·Biochemistry·Kenneth J Woycechowsky, Ronald T Raines
Dec 24, 2008·Biochemistry·Danny RamadanColin Thorpe
Mar 23, 1999·The Biochemical Journal·D M Ferrari, H D Söling
Mar 18, 2009·The Journal of Biological Chemistry·Michele L ForsterBilly Tsai
Feb 24, 2009·The Journal of Biological Chemistry·Jai-Hyun KimHiram F Gilbert
Sep 19, 2003·Antioxidants & Redox Signaling·Elizabeth A Kersteen, Ronald T Raines
Sep 22, 2006·Antioxidants & Redox Signaling·Agnes GörlachThomas Kietzmann
Jun 15, 2006·Antioxidants & Redox Signaling·Carolyn S Sevier, Chris A Kaiser
Apr 25, 2009·Antioxidants & Redox Signaling·Kwonyoon KangKwangseog Ahn
May 30, 2009·Antioxidants & Redox Signaling·Feras Hatahet, Lloyd W Ruddock
Apr 17, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Shoko HashimotoSusumu Imaoka
Aug 6, 2008·Critical Care : the Official Journal of the Critical Care Forum·Mian ZhouPing Wang
Oct 20, 2005·Journal of Bioscience and Bioengineering·T HaradaK Kato
Aug 2, 2014·PloS One·Shang YangJizhong Lou
Sep 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Gayathri GopalanSheng Luan
Feb 4, 2014·Antioxidants & Redox Signaling·Jun Lu, Arne Holmgren
Feb 1, 1997·Molecular Biotechnology·N Darby, T E Creighton
Sep 2, 2004·Cold Spring Harbor Symposia on Quantitative Biology·A D WestonL Hood
Jan 13, 2000·FEBS Letters·H OkudoM Kito
Feb 5, 2013·Journal of Molecular Biology·Andreas FunknerDavid M Ferrari
Oct 27, 2015·ACS Medicinal Chemistry Letters·Anna Kaplan, Brent R Stockwell
Apr 30, 2003·Protein Science : a Publication of the Protein Society·Margherita RuoppoloPeppi Koivunen
Sep 26, 2007·The FEBS Journal·Feras Hatahet, Lloyd W Ruddock
Feb 28, 2008·Chembiochem : a European Journal of Chemical Biology·Griselda HernándezDavid M LeMaster

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.