PMID: 9428688Jan 15, 1998Paper

Functional properties of the quinol oxidase from Acidianus ambivalens and the possible catalytic role of its electron donor--studies on the membrane-integrated and purified enzyme

European Journal of Biochemistry
A GiuffrèM Brunori

Abstract

The aa3 quinol oxidase has been purified from the thermoacidophilic archaea Acidianus ambivalens as a three-redox-centers enzyme. The functional properties of this oxidase both as purified and in its most integral form (i.e. in native membranes and in intact cells) were investigated by stopped-flow spectrophotometry. The results suggest that the enzyme interacts in vivo with a redox-active molecule, which favours the electron entry via heme a and provides the fourth electron demanded for catalysis. We observe that the purified enzyme has two hemes with apparent redox potentials 215 +/- 20 mV and 415 +/- 20 mV at pH 5.4, showing redox-Bohr effect, and a heme a3-CuB center with an affinity for carbon monoxide (Ka = 5.7 x 10(4) M(-1) at 35 degrees C) much lower than that reported for the mammalian enzyme (Ka = 4 x 10(6) M(-1) at 20 degrees C). The reduction by dithionite is fast and monophasic when the quinol oxidase is in the native membranes, whereas it is slow and biphasic in the purified enzyme (with heme a3 being reduced faster than heme a). The oxygen reaction of the reduced purified enzyme is fast (few milliseconds), but yields an intermediate (likely ferryl) clearly different from the fully oxidized enzyme. In contrast, th...Continue Reading

References

Mar 26, 1992·Nature·G T Babcock, M Wikström
Jan 1, 1983·The Biochemical Journal·G D JonesP Sarti
Mar 1, 1995·Biophysical Chemistry·F MalatestaM Brunori
Apr 26, 1995·Biochimica Et Biophysica Acta·O Einarsdóttir
Jan 15, 1995·European Journal of Biochemistry·M TeixeiraW R Hagen
Jun 1, 1994·The Biochemical Journal·N J WatmoughA J Thomson
Aug 15, 1994·European Journal of Biochemistry·M LübbenM Saraste
Apr 30, 1993·Biochemical and Biophysical Research Communications·B C Hill
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·A PuustinenM Wikstrom
Jun 13, 1996·Biochimica Et Biophysica Acta·G L Liao, G Palmer
Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·M I VerkhovskyM Wikström
Dec 18, 1996·Biochimica Et Biophysica Acta·G Schäfer
Mar 1, 1963·The Biochemical Journal·Q H GIBSON, C GREENWOOD

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Citations

Sep 16, 2005·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Tiago M BandeirasNinian J Blackburn
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·T K DasD L Rousseau
Feb 17, 2001·Biochemical and Biophysical Research Communications·R S LemosM Teixeira
Nov 10, 2006·Mikrobiologiia·G I KaravaĭkoT F Kondrat'eva
Apr 22, 2004·Biochimica Et Biophysica Acta·Tapan Kanti DasDenis L Rousseau
Dec 15, 2017·Environmental Science and Pollution Research International·Preeti Ranawat, Seema Rawat
Aug 26, 1998·Biochemical and Biophysical Research Communications·C M Gomes, M Teixeira
Apr 27, 1999·Biochimica Et Biophysica Acta·C M GomesS Anemüller
Sep 8, 1999·Microbiology and Molecular Biology Reviews : MMBR·G SchäferV Müller
Sep 21, 2004·Biophysical Journal·Bruno L VictorCláudio M Soares
Sep 30, 2005·Journal of the American Chemical Society·Smilja TodorovicDaniel H Murgida

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