Functional proteolytic complexes of the human mitochondrial ATP-dependent protease, hClpXP.

The Journal of Biological Chemistry
Sung Gyun KangMichael R Maurizi

Abstract

Human mitochondrial ClpP (hClpP) and ClpX (hClpX) were separately cloned, and the expressed proteins were purified. Electron microscopy confirmed that hClpP forms heptameric rings and that hClpX forms a hexameric ring. Complexes of a double heptameric ring of hClpP with hexameric hClpX rings bound on each side are stable in the presence of ATP or adenosine 5'-(3-thiotriphosphate) (ATPgammaS), indicating that a symmetry mismatch is a universal feature of Clp proteases. hClpXP displays both ATP-dependent proteolytic activity and ATP- or ATPgammaS-dependent peptidase activity. hClpXP cannot degrade lambdaO protein or GFP-SsrA, specific protein substrates recognized by Escherichia coli (e) ClpXP. However, eClpX interacts with hClpP, and, when examined by electron microscopy, the resulting heterologous complexes are indistinguishable from homologous eClpXP complexes. The hybrid eClpX-hClpP complexes degrade eClpX-specific protein substrates. In contrast, eClpA can neither associate with nor activate hClpP. hClpP has an extra C-terminal extension of 28 amino acids. A mutant lacking this C-terminal extension interacts more tightly with both hClpX and eClpX and shows enhanced enzymatic activities but still does not interact with eClpA....Continue Reading

References

Dec 1, 1992·Microbiological Reviews·S Gottesman, M R Maurizi
Apr 1, 1989·European Journal of Biochemistry·G von HeijneR G Herrmann
Oct 1, 1995·Genes & Development·I LevchenkoT A Baker
Jan 1, 1994·Methods in Enzymology·M R MauriziS H Kim
Jan 1, 1996·Journal of Structural Biology·B L TrusA C Steven
Jan 1, 1995·Cold Spring Harbor Symposia on Quantitative Biology·S GottesmanM Maurizi
Dec 15, 1996·Experientia·T Langer, W Neupert
Apr 1, 1997·Trends in Biochemical Sciences·C K SuzukiG Schatz
Nov 25, 1998·FEBS Letters·L van DyckT Langer
Oct 20, 1999·Journal of Molecular Biology·M R de SagarraJ G Casta ñ
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·S K SinghM R Maurizi
Sep 26, 2000·Mammalian Genome : Official Journal of the International Mammalian Genome Society·T J CorydonP Bross
Feb 24, 2001·Cellular and Molecular Life Sciences : CMLS·L Van Dyck, T Langer

❮ Previous
Next ❯

Citations

Apr 28, 2011·ACS Chemical Biology·Jennifer FishovitzIrene Lee
Apr 12, 2012·The Journal of Biological Chemistry·Kayoko KitaTakafumi Ochi
Aug 12, 2009·Journal of Biochemistry·Takashi Tatsuta
Jun 2, 2011·Cold Spring Harbor Perspectives in Biology·Michael J BakerThomas Langer
Jul 10, 2007·Journal of Bacteriology·Jennifer S ChoyA Wali Karzai
Feb 5, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Kaye N TruscottDavid A Dougan
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Susan Gottesman
Apr 9, 2013·Journal of Proteomics·Eirini Lionaki, Nektarios Tavernarakis
May 15, 2007·FEBS Letters·Angela Yeou Hsiung Yu, Walid A Houry
Dec 18, 2015·Biochimica Et Biophysica Acta·Yang Zhang, Michael R Maurizi
Nov 26, 2015·ACS Chemical Biology·Malte GerschStephan A Sieber
May 13, 2008·Journal of Molecular Biology·Dong Young Kim, Kyeong Kyu Kim
Dec 17, 2009·Protein Science : a Publication of the Protein Society·Tahmeena ChowdhuryTania A Baker
Nov 4, 2004·Journal of Structural Biology·Sung Gyun KangBijan Ahvazi
May 10, 2006·Journal of Structural Biology·Agnieszka Szyk, Michael R Maurizi
Dec 15, 2010·Molecular and Cellular Endocrinology·Andrew MidzakVassilios Papadopoulos
Nov 29, 2011·Biochimica Et Biophysica Acta·Sundararajan VenkateshCarolyn K Suzuki
Feb 14, 2004·Structure·Michael R Maurizi, Di Xia
Jun 20, 2012·Journal of Structural Biology·Bradley R LowthDavid A Dougan
Jun 19, 2012·Biochimica Et Biophysica Acta·Wolfgang Voos
Jan 16, 2007·Molecular Cell·Christopher M FarrellRobert T Sauer
Jul 31, 2012·Experimental Cell Research·Katsumi KasashimaHitoshi Endo
Feb 13, 2018·Proceedings of the National Academy of Sciences of the United States of America·Felipe ParedesAlejandra San Martin
Sep 7, 2017·Proceedings of the National Academy of Sciences of the United States of America·Yvette Y YienBarry H Paw
Aug 16, 2018·Chemical Communications : Chem Comm·Thomas F GronauerStephan A Sieber
Aug 22, 2018·Angewandte Chemie·Matthias StahlStephan A Sieber
Jul 16, 2005·Stress : the International Journal on the Biology of Stress·Alberto J L Macario, Everly Conway de Macario
Aug 2, 2007·Molecular Systems Biology·Chris GrillyJeff Hasty
May 5, 2005·The Journal of Biological Chemistry·Gabriela OndrovicováCarolyn K Suzuki
Jun 5, 2010·Organic & Biomolecular Chemistry·Kenneth KnottWebster L Santos
Jun 15, 2007·Critical Reviews in Biochemistry and Molecular Biology·Mirko Koppen, Thomas Langer
Feb 20, 2015·Nature Communications·Malte GerschStephan A Sieber
Nov 1, 2016·The EMBO Journal·Karolina SzczepanowskaAleksandra Trifunovic

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.