Functional proteomics establishes the interaction of SIRT7 with chromatin remodeling complexes and expands its role in regulation of RNA polymerase I transcription.

Molecular & Cellular Proteomics : MCP
Yuan-Chin TsaiIleana M Cristea

Abstract

Among mammalian sirtuins, SIRT7 is the only enzyme residing in nucleoli where ribosomal DNA is transcribed. Recent reports established that SIRT7 associates with RNA Pol I machinery and is required for rDNA transcription. Although defined by its homology to the yeast histone deacetylase Sir2, current knowledge suggests that SIRT7 itself has little to no deacetylase activity. Because only two SIRT7 interactions have been thus far described: RNA Pol I and upstream binding factor, identification of proteins and complexes associating with SIRT7 is critical to understanding its functions. Here, we present the first characterization of SIRT7 interaction networks. We have systematically investigated protein interactions of three EGFP-tagged SIRT7 constructs: wild type, a point mutation affecting rDNA transcription, and a deletion mutant lacking the predicted coiled-coil domain. A combinatorial proteomics and bioinformatics approach was used to integrate gene ontology classifications, functional protein networks, and normalized abundances of proteins co-isolated with SIRT7. The resulting refined proteomic data set confirmed SIRT7 interactions with RNA Pol I and upstream binding factor and highlighted association with factors involved i...Continue Reading

References

Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·R RasmussenT Alber
Jan 1, 1997·Genes & Development·S Strahl-BolsingerM Grunstein
Jan 15, 1997·Genes & Development·J S Smith, J D Boeke
Jan 15, 1997·Genes & Development·M BrykM J Curcio
Jun 30, 2000·Biochemical and Biophysical Research Communications·R A Frye
Nov 14, 2002·The Biochemical Journal·Annemieke J M de RuijterAndré B P van Kuilenburg
Nov 25, 2003·Analytical Chemistry·Alexey I NesvizhskiiRuedi Aebersold
Jun 11, 2004·Annual Review of Biochemistry·Gil Blander, Leonard Guarente
Feb 22, 2005·Nature Cell Biology·Azadeh ArabiAnthony P H Wright
Aug 5, 2005·Molecular Biology of the Cell·Eriko MichishitaIzumi Horikawa
Aug 27, 2005·Current Opinion in Chemical Biology·John M Denu
Sep 13, 2005·Molecular & Cellular Proteomics : MCP·Ileana M CristeaMichael P Rout
Oct 11, 2005·Journal of Proteome Research·Alan J TackettBrian T Chait
Jan 28, 2006·Cell·Raul MostoslavskyFrederick W Alt
Apr 11, 2006·The Journal of Biological Chemistry·Erica CavellánAnn-Kristin Ostlund Farrants
Apr 19, 2006·Genes & Development·Ethan FordLeonard Guarente
Jun 8, 2006·Annual Review of Biochemistry·Anthony A SauveJef D Boeke
Sep 2, 2006·Journal of Proteome Research·Boris ZybailovMichael P Washburn
Nov 14, 2006·Nature Structural & Molecular Biology·Janet G YangGeeta J Narlikar
Dec 16, 2006·Cellular and Molecular Life Sciences : CMLS·T MossV Stefanovsky
Apr 15, 2008·Human Mutation·Richard J GibbonsJoanne Traeger-Synodinos
Jan 29, 2009·Journal of Cell Science·Alice GrobValentina Sirri

❮ Previous
Next ❯

Citations

Sep 13, 2013·Journal of Proteome Research·Erin KaltenbrunFrank L Conlon
Jun 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Tuo LiIleana M Cristea
Oct 23, 2012·The Journal of Biological Chemistry·Jessica L FeldmanJohn M Denu
Jun 14, 2013·Molecular Biology of the Cell·Flávia Gerelli GhiraldiniMaria Luiza Silveira Mello
Dec 15, 2012·PloS One·Oxana PolyakovaRoberto Solari
Nov 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Donald S KirkpatrickKlaus P Hoeflich
Mar 26, 2015·Chemical Reviews·Hui Jing, Hening Lin
Dec 2, 2014·Cellular Signalling·Shashi KiranGayatri Ramakrishna
Jan 29, 2016·Expert Review of Proteomics·Krystal K Lum, Ileana M Cristea
Feb 26, 2016·ACS Chemical Biology·Zhen TongHening Lin
May 12, 2015·Methods : a Companion to Methods in Enzymology·Anna A Georges, Lori Frappier
Jul 15, 2015·Molecular and Cellular Biochemistry·Jarmila Nahálková
Feb 6, 2015·Frontiers in Cell and Developmental Biology·Bor Luen Tang
Aug 12, 2016·Nature Communications·Lianhui SunChuangui Wang
Jun 26, 2012·BioFactors·José M Villalba, Francisco J Alcaín
Sep 17, 2014·The FEBS Journal·Laia Bosch-Presegué, Alejandro Vaquero
Mar 12, 2015·Proteomics·Daniell L RowlesIleana M Cristea
Mar 5, 2016·Structure·Nawsad AlamOra Schueler-Furman
Aug 26, 2015·Biochimica Et Biophysica Acta·Ninu Poulose, Raghavan Raju
Feb 3, 2016·The Journal of Physiology·David L Prole, Colin W Taylor
Apr 21, 2017·Nucleic Acids Research·Maximilian F BlankIngrid Grummt
Jan 18, 2017·Medicinal Research Reviews·Matthias SchiedelManfred Jung
Apr 8, 2016·Molecular & Cellular Proteomics : MCP·Lukasz P SlomnickiMichal Hetman
Oct 29, 2013·Molecular & Cellular Proteomics : MCP·Yana V Miteva, Ileana M Cristea
Dec 15, 2015·Molecular & Cellular Proteomics : MCP·Sophie S B GiguèreIleana M Cristea
Oct 12, 2013·Molecular & Cellular Proteomics : MCP·Yuan-Chin TsaiIleana M Cristea
Aug 10, 2016·Molecular & Cellular Proteomics : MCP·Hanna G Budayeva, Ileana M Cristea
Sep 12, 2017·Antioxidants & Redox Signaling·Chandra K SinghNihal Ahmad

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.