Functional proteomics of resveratrol-induced colon cancer cell apoptosis: caspase-6-mediated cleavage of lamin A is a major signaling loop

Proteomics
Shao Chin LeeS Pervaiz

Abstract

The study investigated the molecular basis of resveratrol (RSV)-evoked apoptosis in four (Bax+/-, Bax-/-, p53+/+, and p53-/-) HCT116 colon cancer cell lines. RSV induced apoptosis in all the cells in a dose-dependent manner; however, Bax+/- and p53+/+ cells were more susceptible than their knockout counterparts (Bax-/- and p53-/-, respectively). Using Bax+/- cells as a model, proteomic analysis revealed four RSV-responsive events: fragmentation of lamin A/C protein; increase in concentration of a more basic isoelectric variant of the ribosomal protein P0; and decrease in concentration of dUTPase as well as stathmin 1. Lamin A cleavage in response to RSV treatment was confirmed using Western blot analysis. Caspase-6 was activated, which was evidenced by cleavage and accumulation in active form of caspase-6 as well as upregulation of the protease activity. RSV-elicited lamin A cleavage and apoptosis were entirely abrogated by the peptide inhibitors of caspase-6. Likewise, partial knockdown of caspase-6 expression using small interfering RNA resulted in significant inhibition of RSV-elicited lamin A cleavage and apoptosis. Furthermore, the lower apoptosis sensitivity of the knockout cells (Bax-/- and p53-/-) correlated with the re...Continue Reading

References

Dec 1, 1996·The Journal of Cell Biology·L RaoE White
Oct 11, 2001·Oncogene·S PervaizM V Clément
Mar 13, 2002·The Journal of Clinical Endocrinology and Metabolism·Ai ShihPaul J Davis
Jun 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Timothy K MacLachlan, Wafik S El-Deiry
Oct 18, 2002·BMC Cancer·Mojgan Mahyar-RoemerKlaus Roemer
Jan 30, 2003·European Journal of Cell Biology·Jos L V BroersFrans C S Ramaekers
Nov 5, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shazib Pervaiz
Sep 3, 2008·Science in China. Series C, Life Sciences·Shaochin Lee, Garth Cooper

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Citations

Feb 20, 2007·Journal of Bioenergetics and Biomembranes·Andrea Lisa Holme, Shazib Pervaiz
May 29, 2013·Fish & Shellfish Immunology·Chenglong JiHongjian Lu
Jun 25, 2008·The British Journal of Nutrition·Siegfried KnasmüllerKarl-Heinz Wagner
May 13, 2009·Antioxidants & Redox Signaling·Shazib Pervaiz, Andrea Lisa Holme
Apr 7, 2010·Antioxidants & Redox Signaling·Ivan Cherh Chiet LowShazib Pervaiz
Nov 23, 2013·PloS One·Nelly GodefroyAndréa C LeBlanc
Jun 1, 2012·The Journal of Nutrition·Donato F Romagnolo, John A Milner
Feb 9, 2007·Pharmacogenomics·Cindy D Davis
Jun 14, 2008·Cancer Letters·Joydeb Kumar Kundu, Young-Joon Surh
Jul 1, 2007·Proteomics. Clinical Applications·Efisio PuxedduLuciano Binaglia
Sep 12, 2007·International Journal of Cancer. Journal International Du Cancer·Ze TianIsaac S Kohane
Dec 21, 2012·Molecular Carcinogenesis·Antoine MénoretDaniel W Rosenberg
Jan 18, 2007·Nitric Oxide : Biology and Chemistry·Simon W Rabkin, Shaun S Klassen
Dec 21, 2006·Nutritional Neuroscience·Minna L Hancock, Robert R Miller
Dec 4, 2014·PloS One·Bénédicte FoveauAndréa C LeBlanc
Sep 13, 2016·Ecotoxicology and Environmental Safety·Xiaoli LiuJunbao Yu
Nov 12, 2016·Nutrients·Aline Renata PavanJean Leandro Dos Santos
Jul 4, 2006·Physiological Reviews·J L V BroersC J Hutchison
Apr 7, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Priya D A IssureeAlirio J Melendez
Jan 1, 2008·Personalized Medicine·Nasséra ChalabiYves-Jean Bignon

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