Functional proteomics of synaptic plasma membrane ATP-ases of rat hippocampus: effect of l-acetylcarnitine and relationships with Dementia and Depression pathophysiology

European Journal of Pharmacology
Federica FerrariRoberto Federico Villa

Abstract

Synaptic energy state and mitochondrial dysfunction are crucial factors in many brain pathologies. l-acetylcarnitine, a natural derivative of carnitine, improves brain energy metabolism, and has been proposed for the Therapy of many neurological and psychiatric diseases. The effects of the drug on the maximum rate (Vmax) of enzymatic activities related to hippocampal synaptic energy utilization were evaluated, in the perspective of its employment for Dementias and Depression Therapy. Two types of synaptic plasma membranes (SPM1 and SPM2) were isolated from the hippocampus of rats treated with l-acetylcarnitine (30 and 60mg/kg i.p., 28 days, 5 days/week). Acetylcholinesterase (AChE); Na(+), K(+), Mg(2+)-ATP-ase; ouabain-insensitive Mg(2+)-ATP-ase; Na(+), K(+)-ATP-ase; Ca(2+), Mg(2+)-ATP-ase activities were evaluated. In control animals, enzymatic activities were differently expressed in SPM1 , being the evaluated enzymatic activities higher in SPM2. Subchronic treatment with l-acetylcarnitine (i) did not modify AChE on both SPMs; (ii) increased Na(+), K(+), Mg(2+)-ATP-ase, ouabain-insensitive Mg(2+)-ATP-ase and Na(+), K(+)-ATP-ase at the dose of 30 and 60mg/kg on SPM1 and SPM2; (iii) increased Ca(2+), Mg(2+)-ATP-ase activity on ...Continue Reading

References

Mar 1, 1978·Analytical Biochemistry·D LeBelA R Beaudoin
May 1, 1986·Journal of Neurochemistry·S T PalayoorD J Bernard
Dec 3, 1971·Biochimica Et Biophysica Acta·C W Cotman, D A Matthews
Feb 1, 1974·Journal of Neurochemistry·J W GurdW J Moore
Feb 1, 1983·Journal of Neurochemistry·N R SimsA N Davison
Jan 1, 1995·Neurobiology of Aging·J W PettegrewR J McClure
Dec 1, 1995·Journal of Clinical Psychopharmacology·L M KoranL W Lazarus
Dec 24, 1997·Annals of the New York Academy of Sciences·M G López Ordieres, G Rodríguez de Lores Arnaiz
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·T M HagenB N Ames
Jun 6, 2002·Bipolar Disorders·Jay W PettegrewRichard J McClure
Aug 22, 2003·Molecular Psychiatry·A MorettiR F Villa
Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE
Nov 5, 2003·Biopharmaceutics & Drug Disposition·Akihiro InanoIkumi Tamai
Jan 11, 2005·Neurotoxicity Research·J AvilaJ J Lucas
Sep 20, 2008·European Journal of Pharmacology·Roberta EpisMonica Di Luca
Jan 15, 2009·European Journal of Nuclear Medicine and Molecular Imaging·Lisa MosconiMony J de Leon
Jan 30, 2010·Journal of Alzheimer's Disease : JAD·Yang-Yang YinLing-Qiang Zhu
Feb 8, 2011·CNS & Neurological Disorders Drug Targets·A MorettiR F Villa
Oct 5, 2012·Fundamental & Clinical Pharmacology·Li-Nan ZhangZi-Bin Gao
Feb 6, 2013·Proceedings of the National Academy of Sciences of the United States of America·Carla NascaFerdinando Nicoletti
Mar 1, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Angelo Demuro, Ian Parker
May 15, 2013·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Bruna CuccurazzuMariagrazia Grilli
Jul 25, 2013·Frontiers in Physiology·Steffany A L BennettDaniel Figeys
Dec 10, 2013·European Journal of Pharmacology·Shreaya Chakroborty, Grace E Stutzmann

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Citations

Jun 9, 2016·Neuroscience·Roberto Federico VillaFabio Tascedda
Dec 22, 2017·International Journal of Molecular Sciences·Santina ChiechioMariagrazia Grilli
May 24, 2017·Aging Clinical and Experimental Research·G SergiE Manzato
Mar 14, 2020·Pharmacology & Therapeutics·Andrea Carlo RossettiRaffaella Molteni
Jul 17, 2021·Neural Regeneration Research·Federica FerrariRoberto Federico Villa

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