Functional reconstitution and characterization of the Arabidopsis Mg(2+) transporter AtMRS2-10 in proteoliposomes

Biochimica Et Biophysica Acta
Sumio IshijimaIkuko Sagami

Abstract

Magnesium (Mg(2+)) plays critical role in many physiological processes. The mechanism of Mg(2+) transport has been well documented in bacteria; however, less is known about Mg(2+) transporters in eukaryotes. The AtMRS2 family, which consists of 10 Arabidopsis genes, belongs to a eukaryotic subset of the CorA superfamily proteins. Proteins in this superfamily have been identified by a universally conserved GlyMetAsn motif and have been characterized as Mg(2+) transporters. Some members of the AtMRS2 family, including AtMRS2-10, may complement bacterial mutants or yeast mutants that lack Mg(2+) transport capabilities. Here, we report the purification and functional reconstitution of AtMRS2-10 into liposomes. AtMRS2-10, which contains an N-terminal His-tag, was expressed in Escherichia coli and solubilized with sarcosyl. The purified AtMRS2-10 protein was reconstituted into liposomes. AtMRS2-10 was inserted into liposomes in a unidirectional orientation. Direct measurement of Mg(2+) uptake into proteoliposomes revealed that reconstituted AtMRS2-10 transported Mg(2+) without any accessory proteins. Mutation in the GMN motif, M400 to I, inactivated Mg(2+) uptake. The AtMRS2-10-mediated Mg(2+) influx was blocked by Co(III)hexamine, a...Continue Reading

References

Jun 11, 1998·Molecular Microbiology·R L Smith, M E Maguire
Apr 5, 2000·The Journal of Biological Chemistry·L M KucharskiM E Maguire
Dec 26, 2001·The Plant Cell·L LiS Luan
Sep 24, 2005·Molecular Genetics and Genomics : MGG·Volker KnoopKatrin Weyand
Dec 6, 2005·Current Genetics·Jong-Min Lee, Richard C Gardner
Apr 7, 2006·Nature·Vladimir V LuninChristopher M Koth
May 25, 2006·Frontiers in Bioscience : a Journal and Virtual Library·Michael E Maguire
Aug 12, 2006·The EMBO Journal·Jian Payandeh, Emil F Pai
Jul 11, 2007·Cellular and Molecular Life Sciences : CMLS·D Niegowski, S Eshaghi
Sep 11, 2007·Biophysical Journal·Rainer SchindlRudolf J Schweyen
Dec 20, 2008·Journal of Integrative Plant Biology·Dan-Dan MaoSheng Luan

❮ Previous
Next ❯

Citations

Sep 26, 2015·International Journal of Molecular Sciences·Natsuko I Kobayashi, Keitaro Tanoi
Jan 15, 2015·Biochimica Et Biophysica Acta·Yixian CuiBing Zhou
Feb 20, 2013·Metallomics : Integrated Biometal Science·Christian HermansNathalie Verbruggen
Dec 10, 2019·Plant Communications·Ren-Jie TangSheng Luan
Jun 28, 2021·International Journal of Biological Macromolecules·Artem StetsenkoAlbert Guskov

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.