PMID: 8953382Dec 1, 1996Paper

Functional reconstitution of photosystem I reaction center from cyanobacterium Synechocystis sp PCC6803 into liposomes using a new reconstitution procedure

Journal of Bioenergetics and Biomembranes
J CladeraM Duñach

Abstract

Photosystem I reaction center from the cyanobacterium Synechocystis sp PCC6803 was reconstituted into phosphatidylcholine/phosphatidic acid liposomes. Liposomes prepared by reversephase evaporation were treated with various amounts of different detergents and protein incorporation was analyzed at each step of the solubilization process. After detergent removal the activities of the resulting proteoliposomes were measured. The most efficient reconstitution was obtained by insertion of the protein complex into preformed liposomes destabilized by saturating amounts of octylglucoside. In the presence of N-methylphenazonium methosulfate and ascorbic acid, liposomes containing the reaction center catalyzed a light-dependent net H+ uptake as measured by the 9-aminoacridine fluorescence quenching and the pH meter. An important benefit of the new reconstitution procedure is that it produces a homogeneous population of large-size proteoliposomes with a low ionic permeability and with a majority inwardly directed H+ transport activity. In optimal conditions, a light-induced delta pH of about 1.8 units could be sustained at 20 degrees C in the presence of valinomycin. In the absence of valinomycin, a "back-pressure" effect of an electrical...Continue Reading

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Citations

Oct 24, 2002·European Journal of Biochemistry·François DevesaYannick Poquet
Aug 25, 2005·IEEE Transactions on Nanobioscience·Tanya KuritzElias Greenbaum
Apr 26, 2005·Biophysical Chemistry·Zhenle YangTingyun Kuang
Jun 17, 2008·IEEE Transactions on Nanobioscience·C P PennisiK Yoshida
Apr 14, 2016·The Journal of Membrane Biology·Hanieh NiroomandBamin Khomami
Nov 25, 2000·Biochimica Et Biophysica Acta·M OllivonM Paternostre
Jul 9, 2004·Biophysical Journal·Philippe GirardPatricia Bassereau
Nov 3, 2021·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Wakana SatoKatarzyna P Adamala

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