PMID: 2492790Feb 15, 1989Paper

Functional reconstitution of prokaryote and eukaryote membrane proteins

Archives of Biochemistry and Biophysics
P C Maloney, S V Ambudkar

Abstract

A new strategy for the functional reconstitution of membrane proteins is described. This approach introduces a new class of protein stabilizing agents--osmolytes--whose presence at high concentration (10-20%) during detergent solubilization prevents the inactivations that normally occur when proteins are extracted from natural membranes. Osmolytes that act in this way include compounds such as glycerol and higher polyols (erythritol, xylitol, sorbitol), sugars (glucose, trehalose), and certain amino acids (glycine, proline, betaine). The beneficial effects of osmolytes are documented by reconstitution of a variety of prokaryote and eukaryote membrane proteins, including several proton- and calcium-motive ATPases, cation- and anion-linked solute carriers (symport and antiport), and a membrane-bound hydrolase from endoplasmic reticulum. In all cases, the presence of 20% glycerol or other osmolyte during detergent solubilization led to 10-fold or more increased specific activity in proteoliposomes. These positive effects did not depend on use of any specific detergent for protein solubilization, nor on any particular method of reconstitution, but for convenience most of the work reported here has used octylglucoside as the solubil...Continue Reading

References

Dec 1, 1979·Archives of Biochemistry and Biophysics·E RackerJ Telford
Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·M Kasahara, P C Hinkle
Jan 1, 1986·Reviews of Physiology, Biochemistry and Pharmacology·H Koepsell
Jun 14, 1985·Biochemical and Biophysical Research Communications·S V Ambudkar, P C Maloney
Mar 1, 1985·Biophysical Journal·T Arakawa, S N Timasheff
Feb 1, 1972·Journal of Bacteriology·E R Kashket, T H Wilson
May 1, 1973·Analytical Biochemistry·P W Holloway
Apr 1, 1984·Journal of Bacteriology·P C MaloneyL Schiller
Sep 24, 1982·Science·P H YanceyG N Somero

❮ Previous
Next ❯

Citations

Nov 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P C Maloney
Aug 1, 1990·Journal of Bioenergetics and Biomembranes·P C Maloney
Feb 1, 1995·Journal of Bioenergetics and Biomembranes·S V Ambudkar
Nov 1, 1993·FEMS Microbiology Reviews·G I VeldW N Konings
Mar 1, 1995·The International Journal of Biochemistry & Cell Biology·V VachonR Béliveau
Mar 22, 2001·Advanced Drug Delivery Reviews·T ArakawaJ F Carpenter
Oct 23, 1998·Biochimie·J ChopineauM Ollivon
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·L BishopG F Ames
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A J Driessen, W Wickner
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S V AmbudkarI Pastan
Aug 1, 1990·Molecular Microbiology·A Varadhachary, P C Maloney
Sep 29, 2011·PloS One·Chi-Jiunn PanJanice Y Chou
Dec 3, 2014·Biochimica Et Biophysica Acta·Kim S HorgerMichael Mayer
Jun 11, 2014·Critical Reviews in Food Science and Nutrition·Salim Ur-RehmanMian Anjum Murtaza
Sep 1, 1995·Current Eye Research·L J Jarvis, C F Louis
Feb 22, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·I S Ambudkar
Jul 14, 2018·The Plant Journal : for Cell and Molecular Biology·Kristian Mark P CaldoM Joanne Lemieux
Mar 14, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shih-Yin ChenJanice Y Chou
Dec 5, 1998·The Journal of Biological Chemistry·M C Fann, P C Maloney
Jan 6, 2021·Cold Spring Harbor Protocols·Clara L KielkopfIna L Urbatsch
Jun 16, 2004·Biochemistry·J G DumanJ G Forte
Nov 2, 1993·Biochimica Et Biophysica Acta·B Poolman, W N Konings
May 15, 1990·Biochimica Et Biophysica Acta·A Villalobo
May 24, 1990·Biochimica Et Biophysica Acta·I DurieuxR Got
Mar 1, 1990·Microbiological Reviews·P C MaloneyA Varadhachary
Jan 6, 2009·Protein Expression and Purification·Alla KorepanovaMark L Chiu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.