Functional recovery of donation after cardiac death liver graft by continuous machine perfusion preservation in pigs

Transplantation Proceedings
T ShigetaS Enosawa

Abstract

Grafts from donation after cardiac death (DCD) will greatly contribute to the expand the donor pool. However, these grafts may require the development of the preservation methods because of primary nonfunction and severe ischemic bile duct injury. Porcine livers were perfused with a newly developed machine perfusion (MP) system. Each system for the portal vein or the hepatic artery had a roller pump, a flow meter, and a pressure sensor. The livers were perfused with University of Wisconsin (UW)-gluconate at 4°C-6°C for 3 hours after 2 hours simple cold storage (CS). The portal vein flow rate was 0.5 mL/min/g liver (pressure, 10 mm Hg) and the hepatic artery flow rate was 0.2 mL/min/g liver (pressure, 30 mm Hg). Orthotopic liver transplantation was performed in pigs comparing Group 1 (n = 4) procured after acute hemorrhagic shock preserved by MP, Group 2 (n = 3) procured after warm ischemia time (WIT) of 30 minutes with CS preservation, and Group 3 (n = 4) procured with 30 minutes of WIT and MP preservation. Collected effluent aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) levels in the perfusion solution and serum AST and LDH were significantly lower in Group 1. AST and LDH results were lower in Group 3 than G...Continue Reading

References

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Citations

Nov 29, 2013·Transplantation Research·Naoto Matsuno, Eiji Kobayashi
Jan 17, 2014·Langenbeck's Archives of Surgery·Helge Bruns, Peter Schemmer
May 13, 2014·Transplantation Proceedings·N MatsunoH Furukawa
Apr 2, 2014·Hepatobiliary & Pancreatic Diseases International : HBPD INT·Xiao-Bo Chen, Ming-Qing Xu
Mar 4, 2014·The Journal of Surgical Research·Dmitri BezinoverPiotr K Janicki
May 10, 2016·Transplantation Reviews·A S BarbasM Selzner
Apr 3, 2016·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Zhi-Bin ZhangZhi-Jun Zhu
Feb 28, 2017·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Hazel MareckiPaulo N Martins

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