PMID: 8589447Nov 1, 1995Paper

Functional recovery of troponin I in a Drosophila heldup mutant after a second site mutation

Molecular Biology of the Cell
A PradoA Ferrús

Abstract

To identify proteins that interact in vivo with muscle components we have used a genetic approach based on the isolation of suppressors of mutant alleles of known muscle components. We have applied this system to the case of troponin I (TnI) in Drosophila and its mutant allele heldup2 (hdp2). This mutation causes an alanine to valine substitution at position 116 after a single nucleotide change in a constitutive exon. Among the isolated suppressors, one of them results from a second site mutation at the TnI gene itself. Muscles endowed with TnI mutated at both sites support nearly normal myofibrillar structure, perform notably well in wing beating and flight tests, and isolated muscle fibers produce active force. We show that the structural and functional recovery in this suppressor does not result from a change in the stoichiometric ratio of TnI isoforms. The second site suppression is due to a leucine to phenylalanine change within a heptameric leucine string motif adjacent to the actin binding domain of TnI. These data evidence a structural and functional role for the heptameric leucine string that is most noticeable, if not specific, in the indirect flight muscle.

References

Apr 1, 1991·Molecular & General Genetics : MGG·D R DrummondJ C Sparrow
Jan 1, 1967·Progress in Biophysics and Molecular Biology·J W Pringle
Jan 1, 1969·Journal of Ultrastructure Research·A R Spurr
Dec 1, 1967·Journal of Morphology·J Lai-Fook

❮ Previous
Next ❯

Citations

May 10, 2001·Trends in Cardiovascular Medicine·A FerrúsA Hernández-Hernández
May 22, 2001·Molecular Biology of the Cell·B NaimiJ C Sparrow
Mar 23, 1999·The Journal of Cell Biology·W A KronertS I Bernstein
Apr 19, 2007·PloS One·Sarah KnoxScott B Selleck
Dec 18, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J O Vigoreaux
Aug 29, 1998·The Journal of Biological Chemistry·H H PierceB K Kay
Jul 1, 2005·Physiological Reviews·Scott L Hooper, Jeffrey B Thuma
Feb 10, 2021·G3 : Genes - Genomes - Genetics·Miles W DeAngelisRuth I Johnson
Jul 2, 2009·Journal of Cell Science·Virender Kumar SahotaAlberto Ferrús

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.